E2513
ExtrAvidin®-Agarose, high binding
For purification of biotinylated macromolecules and complexes
Synonym(s):
ExtrAvidin® beads, de-glycosylated Avidin
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Available to ship TODAYfromMILWAUKEE | $224.00 |
5 mL | Available to ship TODAYfromMILWAUKEE | $745.00 |
About This Item
$224.00
Available to ship TODAYDetails
form
aqueous suspension, suspension
feature
For purification of biotinylated macromolecules and complexes
concentration
50%
matrix
4% agarose (beaded)
matrix activation
cyanogen bromide
storage temp.
2-8°C
General description
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
Application
Physical form
Legal Information
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This Item | |||
|---|---|---|---|
| description For purification of biotinylated macromolecules and complexes | description buffered aqueous solution | description buffered aqueous solution | description buffered aqueous solution |
| concentration 50% | concentration - | concentration - | concentration - |
| form suspension, aqueous suspension | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| matrix 4% agarose (beaded) | matrix - | matrix - | matrix - |
| matrix activation cyanogen bromide | matrix activation - | matrix activation - | matrix activation - |
| feature For purification of biotinylated macromolecules and complexes | feature - | feature - | feature - |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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