Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

E3403

EZview™ Red Protein G Affinity Gel

Synonym(s):

EZview Affinity Gel, Protein G Affinity Gel

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 mL
Check Cart for Availability
$331.00
5 x 1 mL
Check Cart for Availability
$1,130.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.56
MDL number:
MFCD03456743
Form:
liquid

$331.00

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

liquid

Quality Level

200

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

storage temp.

−20°C

General description

EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
P6486E6779E6406
EZview™ Red Protein G Affinity Gel

Millipore

E3403

EZview™ Red Protein G Affinity Gel

EZview™ Red Protein A Affinity Gel

Millipore

P6486

EZview™ Red Protein A Affinity Gel

EZview™ Red Anti-HA Affinity Gel

Millipore

E6779

EZview™ Red Anti-HA Affinity Gel

EZview™ Red Glutathione Affinity Gel

Millipore

E6406

EZview™ Red Glutathione Affinity Gel

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

form

liquid

form

liquid

form

-

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

capacity

~4 mg binding capacity (rabbit IgG)

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

capacity

-

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

proteins (hemagglutinin)

analyte chemical class(es)

proteins (GST)

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000493026
0000493026
SLCG1583
SLBQ8240V
SLBN8308V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Antibody Support Reagents

Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.

eView Resins
Product Information Sheet - E3403

Instructions

Yuval Malka et al.
Nature communications, 8(1), 2029-2029 (2017-12-13)
The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and
Min Mo et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(46), 19527-19532 (2009-11-06)
The anaphase-promoting complex (APC), or cyclosome, is a ubiquitin ligase with major roles in cell cycle regulation. It is required for mitotic exit, but must be deactivated for the G(1)/S phase transition to occur. APC consists of at least 12
Álvaro Quintanal-Villalonga et al.
EBioMedicine, 53, 102683-102683 (2020-03-03)
Fibroblast growth factor receptor (FGFR)1 and FGFR4 have been associated with tumorigenesis in a variety of tumour types. As a therapeutic approach, their inhibition has been attempted in different types of malignancies, including lung cancer, and was initially focused on
View All Related Papers

Global Trade Item Number

SKUGTIN
E3403-1ML04061838255280
E3403-5X1ML04061833603277

Questions

Reviews

No rating value

Active Filters