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E3403

EZview™ Red Protein G Affinity Gel

Synonyme(s) :

EZview Affinity Gel, Protein G Affinity Gel

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À vous/RéférenceDisponibilitéPrix
1 mL

Disponible dès aujourd'huiDeSt QuentinFallavier

404,00 €
5 x 1 mL
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A propos de cet article

UNSPSC Code:
12352202
NACRES:
NA.56
MDL number:
MFCD03456743
Form:
liquid

404,00 €

Disponible dès aujourd'huiDétails

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form

liquid

Quality Segment

200

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

storage temp.

−20°C

General description

EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and MIT-HClis present at 30 PPM and 0.003% (w/v).
50% slurry in PBS with 50%Glycerol and 0.0015%

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

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Cet article
E6406539322P6486
EZview™ Red Protein G Affinity Gel

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E3403

EZview™ Red Protein G Affinity Gel

EZview™ Red Glutathione Affinity Gel

Millipore

E6406

EZview™ Red Glutathione Affinity Gel

Protein G, Peroxidase Conjugate This Protein G, Peroxidase Conjugate is validated for use in Direct Immunoassay.

Sigma-Aldrich

539322

Protein G, Peroxidase Conjugate

EZview™ Red Protein A Affinity Gel

Millipore

P6486

EZview™ Red Protein A Affinity Gel

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

-

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

form

liquid

form

-

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

analyte chemical class(es)

proteins (GST)

analyte chemical class(es)

-

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

capacity

-

capacity

-

capacity

~4 mg binding capacity (rabbit IgG)

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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