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E8029

E-Toxate™ Endotoxin standard

for endotoxin quantitation

Synonyme(s) :

E. coli 0.55:B5 LPS standard

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1 vial
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97,20 $

A propos de cet article

NACRES:
NA.24
UNSPSC Code:
12352202

97,20 $

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Quality Segment

200

contains

solubilizer

storage temp.

2-8°C

Application

E-Toxate endotoxin standard was used along with the limulus amebocyte lysate assay kit to determine the concentration of endotoxins in isolated samples of human holotransferrin and apotransferrin used to treat primary hepatocyte cultures.

Analysis Note

Standardized against USP reference standard endotoxin (RSE)

Other Notes

A preparation of E. coli 055:B5 lipopolysaccharide
Can be stored at 2-8 °C when stored separately from the E-Toxate kit.

Legal Information

E-Toxate is a trademark of Sigma-Aldrich Co. LLC

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Cet article
VT001796VT002023VT000126
E-Toxate™ Endotoxin standard for endotoxin quantitation

Sigma-Aldrich

E8029

E-Toxate™ Endotoxin standard

Escherichia coli WDCM 00179 Vitroids™ 1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Millipore

VT001796

Escherichia coli WDCM 00179 Vitroids™

Escherichia coli WDCM 00202 Vitroids™ 80-130 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Millipore

VT002023

Escherichia coli WDCM 00202 Vitroids™

Escherichia coli WDCM 00012 Vitroids™ 1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Millipore

VT000126

Escherichia coli WDCM 00012 Vitroids™

description

for endotoxin quantitation

description

1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

description

80-130 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

description

1,000-10,000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

contains

solubilizer

contains

-

contains

-

contains

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  

  1. Hi, For E8029 endotoxin quantification product, 1. Is it a powder or liquid? 2. Solubility in water if it's a powder? How much water is needed to prepare a solution? Thanks,

    1 answer

    1. This product is a lyophilized powder typically used as a standard in the E-Toxate Endotoxin kits. See the link below to review these kits. For use with these kits, the material is reconstituted in endotoxin-free water at 4000UE/mL. Each vial contains a range of 10,000 - 20,000 units which is reported in the lot specific Certificate of Analysis. The mass weight is not determined. However, the amount of powder may be estimated based on the conversion factor of 5 EU per nanogram.
      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/e8029#product-documentation

      Please see the link below to review the E-Toxate Endotoxin Kit products:
      https://www.sigmaaldrich.com/substance/etoxatekit1234598765

      Please see the link below to review the kit protocol with reconstitution instructions for this standard:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/800/et0200-pis-ms.pdf

      Helpful?

  2. What is the amount of the powder in each vial in grams or milligrams?

    1 answer

    1. The product comes as 10000 - 20000 EU Endotoxin per vial. Unfortunately, the exact weight information is not available.

      Helpful?

  3. What is the E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. The endotoxin standard is purified from a crude extract of lipopolysaccharide from E. coli 055:B5 and standardized against the current USP reference standard.  It is reconstituted with an appropriate amount of water to yield a 4000 EU/mL solution.

      Helpful?

  4. How much water should I use to reconstitute my E-Toxate™ Endotoxin standard, Product E8029?

    1 answer

    1. Each batch of endotoxin standard varies slightly in its activity so the amount of water to use may vary from lot to lot.  The certificate of analysis will give the amount of endotoxin units in the vial, so that the end user may calculate the appropriate amount of water to add to the vial in order to reconsitute to a final concentration of 4000 EU/mL.  The certificate of analysis for each lot may be retrieved from our website.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. How is the E-Toxate™ Endotoxin standard, Product E8029, dissolved?

    1 answer

    1. E8029 is reconstituted in endotoxin-free water to a concentration of 4000 EU/mL.  Reconstituted standard solutions can be stored at 2 - 8°C for at least 2 weeks.

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