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F3133

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

Sinônimo(s):

FGF

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Para você/SKUDisponibilidadePreço
10 μg
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R$ 2.504,00

Sobre este item

Número CAS:
62031-54-3
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00166724
Biological source:
bovine pituitary glands
Form:
lyophilized powder
Mol wt:
16.4 kDa
Impurities:
endotoxin, tested

R$ 2.504,00

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Nome do produto

Fibroblast Growth Factor from bovine pituitary, suitable for cell culture

biological source

bovine pituitary glands

Quality Segment

200

form

lyophilized powder

potency

1-100 ng/mL

mol wt

16.4 kDa

packaging

pkg of 10 and 5X10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white to off-white

storage temp.

−20°C

General description

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Biochem/physiol Actions

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Preparation Note

Purified glands by a modification of the method of Gospodarowicz.

Analysis Note

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.
The biological activity is measured using a 3-day MTT assay.

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F8424T5300F1168
Fibroblast Growth Factor from bovine pituitary suitable for cell culture

Sigma-Aldrich

F3133

Fibroblast Growth Factor from bovine pituitary

Fibroblast Growth Factor-4 human FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

F8424

Fibroblast Growth Factor-4 human

Transforming Growth Factor-β2 from porcine platelets powder, suitable for cell culture

Sigma-Aldrich

T5300

Transforming Growth Factor-β2 from porcine platelets

Fibroblast Growth Factor-9 human recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Sigma-Aldrich

F1168

Fibroblast Growth Factor-9 human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

bovine pituitary glands

biological source

human

biological source

Porcine (Platelets)

biological source

human

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

impurities

endotoxin, tested

impurities

<1 EU/μg (LAL test)

impurities

endotoxin, tested

impurities

endotoxin, tested

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Classe de armazenamento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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