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F3665

Lipopolysaccharides from Escherichia coli O111:B4

FITC conjugate

Synonym(s):

LPS

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1 mg
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$154.00
10 mg
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$796.00

About This Item

CAS Number:
93572-42-0
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
MFCD00164401

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biological source

Escherichia coli (O111:B4)

Quality Level

200

conjugate

FITC conjugate

form

lyophilized powder

extent of labeling

2-10 μg FITC per mg LPS

color

yellow to orange

solubility

H2O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

storage temp.

2-8°C

Application

FITC-Lipopolysaccharides (FITC-LPS) may be used to study processes such as the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. It may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.[1][2]
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) yielding a hazy, yellow to orange solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.

Other Notes

To gain a comprehensive understanding of our extensive range of lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Lipopolysaccharides from Escherichia coli O111:B4 FITC conjugate

Sigma-Aldrich

F3665

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O55:B5 FITC conjugate

Sigma-Aldrich

F8666

Lipopolysaccharides from Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O111:B4 purified by gel-filtration chromatography

Sigma-Aldrich

L3012

Lipopolysaccharides from Escherichia coli O111:B4

Lipopolysaccharides from Escherichia coli O111:B4 purified by trichloroacetic acid extraction

Sigma-Aldrich

L4130

Lipopolysaccharides from Escherichia coli O111:B4

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O111:B4)

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

solubility

H2O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

solubility

water: soluble

solubility

water: soluble

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to orange

color

yellow to orange

color

white to faint yellow

color

white to light yellow

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pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
079M4078V
12190104
12190104
0000465489
0000465499

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Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Yannick Boege et al.
Cancer cell, 32(3), 342-359 (2017-09-13)
Concomitant hepatocyte apoptosis and regeneration is a hallmark of chronic liver diseases (CLDs) predisposing to hepatocellular carcinoma (HCC). Here, we mechanistically link caspase-8-dependent apoptosis to HCC development via proliferation- and replication-associated DNA damage. Proliferation-associated replication stress, DNA damage, and genetic
Saurabh Srivastava et al.
Antimicrobial agents and chemotherapy, 57(6), 2457-2466 (2013-03-13)
Temporin L (TempL) is a 13-residue frog antimicrobial peptide that shows moderate bactericidal activity and antiendotoxin properties in macrophages. We envisioned that, due to its very hydrophobic nature, the peptide might fail to show its desired biological properties. It was
Takashi Orimo et al.
International immunology, 31(10), 657-668 (2019-01-29)
Cholera toxin B (CTB) is a subunit of cholera toxin, a bacterial enterotoxin secreted by Vibrio cholerae and also functions as an immune adjuvant. However, it remains unclear how CTB activates immune cells. We here evaluated whether or how CTB
View All Related Papers

Global Trade Item Number

SKUGTIN
F3665-10MG04061833271001
F3665-1MG04061833617144

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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