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Anti-Human IgG2−FITC antibody, Mouse monoclonal

Name: Anti-Human IgG2−FITC antibody, Mouse monoclonal
Brand: SIGMA
Price: 224 USD
Availability: InStock

clone HP-6014, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgG2

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$224.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

HP-6014, monoclonal

Application:

DIBA, PIFA

Citations:

$224.00

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Properties

biological source

mouse

Quality Level

300

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

HP-6014, monoclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

dot immunobinding: 1:32, particle immunofluorescence: 1:16

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and residues inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain light chains paired with the VH and CH1 domains of the heavy chains.

Application

Monoclonal Anti-Human IgG2-FITC antibody produced in mouse has been used in immunohistochemistry.

Biochem/physiol Actions

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Physical form

Solution in 0.01 M phosphate buffered saline, pH8, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8ºC for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	F5016	F0767	F9890
Sigma-Aldrich F4516 Anti-Human IgG2−FITC antibody, Mouse monoclonal	Sigma-Aldrich F5016 Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal	Sigma-Aldrich F0767 Anti-Human IgG1−FITC antibody, Mouse monoclonal	Sigma-Aldrich F9890 Anti-Human IgG4−FITC antibody, Mouse monoclonal
technique(s) dot immunobinding: 1:32, particle immunofluorescence: 1:16	technique(s) dot immunobinding: 1:16, particle immunofluorescence: 1:16	technique(s) dot immunobinding: 1:64, particle immunofluorescence: 1:32	technique(s) dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
isotype IgG1	isotype IgG2a	isotype IgG2a	isotype IgG1
clone HP-6014, monoclonal	clone HP-6017, monoclonal	clone 8c/6-39, monoclonal	clone HP-6025, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000492380

0000472434

0000470981

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Articles

How to Choose a Secondary Antibody

Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.

Global Trade Item Number

SKU	GTIN
F4516-.2ML	04061837516771

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