F4516

Sigma-Aldrich

Monoclonal Anti-Human IgG2−FITC antibody produced in mouse

clone HP-6014, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-Human IgG2
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP-6014, monoclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

dot immunobinding: 1:32
particle immunofluorescence: 1:16

isotype

IgG1

conjugate

FITC conjugate

shipped in

dry ice

storage temp.

−20°C

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and residues inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain light chains paired with the VH and CH1 domains of the heavy chains.

Specificity

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG2-FITC antibody produced in mouse has been used in immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH8, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8ºC for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Human placental Fc receptors and the transmission of antibodies from mother to fetus.
Simister NE and Story CM
Journal of Reproductive Immunology, 37(1) (1997)
Immunobiology (2001)
Antibody structure, instability, and formulation.
Wang W, et al.
Journal of Pharmaceutical Sciences (2007)
Evidence for antibody-mediated pathogenesis in anti-NMDAR
encephalitis associated with ovarian teratoma
Erdem Tuzun, et al.
Acta Neuropathologica, 118(6) (2009)
Aki Kuroki et al.
Kidney international, 68(1), 302-310 (2005-06-16)
The predominant deposition of IgG4 in idiopathic membranous nephropathy indicates that its presence characterizes the systemic immune response of the disease. We analyzed the expressions of CD3, CD19, CD4, and CD8 on peripheral blood mononuclear cells (PBMCs) by flow cytometry...

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