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Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal

Name: Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal
Brand: SIGMA
Price: 141 USD
Availability: InStock

clone HP-6017, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgG (Fc specific)

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$141.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

FITC conjugate

Clone:

HP-6017, monoclonal

Application:

DIBA, PIFA

Citations:

$141.00

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Properties

biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

HP-6017, monoclonal

form

buffered aqueous solution

species reactivity

rabbit, sheep, horse (IgG), goat, human

storage condition

protect from light

technique(s)

dot immunobinding: 1:16, particle immunofluorescence: 1:16

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Application

Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in:

Fluorescent Dot Immunobinding Assay (F-DIBA)
Particle Immunofluorescent Assay (F-IFMA)
flow cytometry

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding.

The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Physical form

Solution in 0.01 M phosphate buffer pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	F4516	F0767	B3773
Sigma-Aldrich F5016 Anti-Human IgG (Fc specific)−FITC antibody, Mouse monoclonal	Sigma-Aldrich F4516 Anti-Human IgG2−FITC antibody, Mouse monoclonal	Sigma-Aldrich F0767 Anti-Human IgG1−FITC antibody, Mouse monoclonal	Sigma-Aldrich B3773 Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate biotin conjugate
species reactivity rabbit, sheep, horse (IgG), goat, human	species reactivity -	species reactivity -	species reactivity rabbit, sheep, horse (IgG), goat, human
technique(s) dot immunobinding: 1:16, particle immunofluorescence: 1:16	technique(s) dot immunobinding: 1:32, particle immunofluorescence: 1:16	technique(s) dot immunobinding: 1:64, particle immunofluorescence: 1:32	technique(s) direct ELISA: 1:80,000
biological source mouse	biological source mouse	biological source mouse	biological source mouse
isotype IgG2a	isotype IgG1	isotype IgG2a	isotype IgG2a

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000543479

0000475634

0000420007

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Global Trade Item Number

SKU	GTIN
F5016-.2ML	04061833618059

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