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F8646

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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0.5 mL
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$354.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162628
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IHC (p)
Citations:
6

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biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody is specific for the Fc fragment of mouse IgG. No cross reactivity is observed with the Fab fragment of mouse IgG. Purified antibody is conjugated to fluorescein isothiocyanate (FITC) in an alkaline reaction and then purified to remove unbound FITC.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody may be used for immunohistochemistry at a working dilution of 1:320 using formalin-fixed, paraffin-embedded sections of human tonsil. Antibody dilution of 1:200 was used to label sections of human brainstems and cervical spinal cords with primary antibody against human Bcl-2 protein. The antibody was also used to label erythrocytes for photon reassignment microscopy and for FACS using melanoma cells.

Biochem/physiol Actions

Binds mouse IgG; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with bovine, equine, or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
F2772F8521F8264
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F8646

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F2772

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F8521

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F8264

Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

technique(s)

flow cytometry: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

technique(s)

direct immunofluorescence: 1:64

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
082M4825V
125M4882V
041M4760
060M4792
118K4826

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Related Content

Data Sheet - F8646

Instructions

K A Muczynski et al.
Journal of immunology (Baltimore, Md. : 1950), 160(7), 3207-3216 (1998-04-08)
We compared HLA class II expression in a human melanoma line (a nonprofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class II expression in an EBV-transformed B lymphoblastoid cell line (a professional APC) from the
J Gysin et al.
Infection and immunity, 67(12), 6596-6602 (1999-11-24)
We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and
M Coulpier et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 16(19), 5897-5904 (1996-10-01)
The molecular events leading to motoneuronal death are still poorly understood. In mammals, the bcl-2 proto-oncogene, which encodes a membrane-associated protein, has been shown to suppress both developmental motoneuronal death and experimental axotomy-induced motoneuronal death. We assessed a potential protective
View All Related Papers

Global Trade Item Number

SKUGTIN
F8646-.5ML04061837517211

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