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G1777

Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

ATF, GDNF

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10 μg
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$246.00

About This Item

MDL number:
MFCD00285867
EC Number:
231-509-8
NACRES:
NA.32
UNSPSC Code:
12352202

$246.00

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biological source

human

Quality Segment

recombinant

expressed in E. coli

form

lyophilized powder

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... GDNF(2668)

General description

Recombinant human Glial Cell Line‑Derived Neurotrophic Factor (GDNF) is expressed in E. coli and supplied as a lyophilized powder. GDNF is a member of the GDNF family of ligands and is used in in vitro research involving neuronal cell culture.

Application

GDNF has been used in neuronal and stem cell culture research applications to support cell survival and differentiation in vitro. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

Biochem/physiol Actions

GDNF is a neurotrophic factor that mediates cellular responses through GDNF family receptor signaling complexes and has been extensively studied in neuronal cell models in vitro. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.
Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Mature human GDNF, a disulfide-linked homo­dimeric glycoprotein, is predicted to contain two 15 kDa polypeptide chains.

Physical form

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.

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Glial Cell Line-derived Neurotrophic Factor human recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

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Glial Cell Line-derived Neurotrophic Factor human

Glial Cell Line-derived Neurotrophic Factor from rat recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Sigma-Aldrich

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Sigma-Aldrich

SRP3309

GDNF human

Ciliary Neurotrophic Factor human CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

C3710

Ciliary Neurotrophic Factor human

description

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

description

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

biological source

rat

biological source

human

biological source

human

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

lyophilized powder

Gene Information

human ... GDNF(2668)

Gene Information

rat ... Gdnf(25453)

Gene Information

human ... GDNF(2668)

Gene Information

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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