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Glial Cell Line-derived Neurotrophic Factor human

Name: Glial Cell Line-derived Neurotrophic Factor human
Brand: SIGMA
Price: 241 USD
Availability: InStock

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):

ATF, GDNF

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10 μg	Check Cart for Availability	$241.00

About This Item

MDL number:

MFCD00285867

EC Number:

231-509-8

NACRES:

NA.32

UNSPSC Code:

12352202

$241.00

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Properties

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

form

lyophilized powder

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

UniProt accession no.

P39905

storage temp.

−20°C

Gene Information

human ... GDNF(2668)

Description

General description

Recombinant human Glial Cell Line‑Derived Neurotrophic Factor (GDNF) is expressed in E. coli and supplied as a lyophilized powder. GDNF is a member of the GDNF family of ligands and is used in in vitro research involving neuronal cell culture.

Application

GDNF has been used in neuronal and stem cell culture research applications to support cell survival and differentiation in vitro. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs).

Biochem/physiol Actions

GDNF is a neurotrophic factor that mediates cellular responses through GDNF family receptor signaling complexes and has been extensively studied in neuronal cell models in vitro. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Mature human GDNF, a disulfide-linked homodimeric glycoprotein, is predicted to contain two 15 kDa polypeptide chains.

Physical form

Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures.

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This Item	B3795	H8416	P3326
Sigma-Aldrich G1777 Glial Cell Line-derived Neurotrophic Factor human	Sigma-Aldrich B3795 Brain-derived neurotrophic factor human	Sigma-Aldrich H8416 Stem Cell Factor human	Sigma-Aldrich P3326 Platelet-Derived Growth Factor-AB human
description recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture	description PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source human	biological source human	biological source human	biological source human
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
impurities endotoxin, tested	impurities ≤1.00 EU/μg Endotoxin level	impurities ≤1 EU/μg	impurities ≤1 EU/μg

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Select a Size

About This Item

$241.00

biological source

Quality Segment

recombinant

form

packaging

storage condition

technique(s)

impurities

color

UniProt accession no.

storage temp.

Gene Information

General description

Application

Biochem/physiol Actions

Physical form

Analysis Note

Compare Similar Items

This Item

Safety Information

Storage Class

wgk

ppe

Documentation

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Glial Cell Line-derived Neurotrophic Factor human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Select a Size

About This Item

G1777-10UG

$241.00

biological source

Quality Segment

recombinant

form

packaging

storage condition

technique(s)

impurities

color

UniProt accession no.

storage temp.

Gene Information

General description

Application

Biochem/physiol Actions

Physical form

Analysis Note

Compare Similar Items

This Item

Safety Information

Storage Class

wgk

ppe

Documentation

Certificates of Analysis (COA)

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