The concentration of antibody in this ascitic fluid is typically in the range of 6 to 10 mg/mL.
I6510
Monoclonal Anti-Human IgE antibody produced in mouse
clone GE-1, ascites fluid
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Synonym(s):
Monoclonal Anti-Human IgE
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| Size/SKU | Availability | Price |
|---|---|---|
0.1 mL | Available to ship TODAYfromMILWAUKEE | $315.00 |
0.25 mL | Available to ship TODAYfromMILWAUKEE | $638.00 |
About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GE-1, monoclonal
Application:
ELISA (i)
Citations:
13
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
GE-1, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:5,000
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Binds human IgE; does not bind human IgG, IgA or IgM.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.
Application
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.
Biochem/physiol Actions
Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells. It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.
Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.
Physical form
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Analysis Note
The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone GE-1, ascites fluid | description affinity isolated antibody, lyophilized powder | description This Human IgE, Myeloma is validated for use as an Immunoassay standard. | description clone GE-1, purified from hybridoma cell culture |
| antibody form ascites fluid | antibody form affinity isolated antibody | antibody form - | antibody form purified from hybridoma cell culture |
| biological source mouse | biological source goat | biological source (Human myeloma) | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate - | conjugate - |
| technique(s) indirect ELISA: 1:5,000 | technique(s) Ouchterlony double diffusion: suitable | technique(s) - | technique(s) immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma, ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating, immunoblotting: suitable |
| isotype IgG2b | isotype - | isotype IgE | isotype IgG2b |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −70°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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