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K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

clone KP-53, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human Kappa Light Chain (Bound and Free)

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0.2 mL
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$371.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162517
Conjugate:
unconjugated
Technique(s):
indirect ELISA: 1:10,000
Application:
ELISA (i)
Citations:
10

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

KP-53

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:10,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Application

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Biochem/physiol Actions

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal clone KP-53, purified from hybridoma cell culture

Sigma-Aldrich

K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse clone HP-6054, ascites fluid

Sigma-Aldrich

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Monoclonal Anti-Human Lambda Light Chains (Bound and Free) antibody produced in mouse

Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

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Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat

Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A7164

Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat

description

clone KP-53, purified from hybridoma cell culture

description

clone HP-6054, ascites fluid

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

indirect ELISA: 1:10,000

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:1,000 using using 2 μg/ml of human IgG myeloma containing lambda light chains as the coating protein, radioimmunoassay: suitable, western blot: suitable

technique(s)

direct ELISA: 1:1,000

technique(s)

direct ELISA: 1:1,000

biological source

mouse

biological source

mouse

biological source

goat

biological source

goat

isotype

IgG1

isotype

IgG2a

isotype

-

isotype

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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