Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

clone KP-53, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human Kappa Light Chain (Bound and Free)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
0.2 mL
Check Cart for Availability
$384.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162517
Conjugate:
unconjugated
Technique(s):
indirect ELISA: 1:10,000
Application:
ELISA (i)
Citations:
10

$384.00

Check Cart for Availability
​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

KP-53

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:10,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Application

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Biochem/physiol Actions

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
AB714K3502F3761
Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal clone KP-53, purified from hybridoma cell culture

Sigma-Aldrich

K4377

Anti-Human Kappa Light Chain (Bound and Free) antibody, Mouse monoclonal

Sigma-Aldrich

Sigma-Aldrich

AB714

Anti-Human Kappa Light Chain (Free and Bound)

Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat affinity isolated antibody, lyophilized powder

Sigma-Aldrich

K3502

Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat

Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F3761

Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody produced in goat

description

clone KP-53, purified from hybridoma cell culture

description

-

description

affinity isolated antibody, lyophilized powder

description

affinity isolated antibody, buffered aqueous solution

antibody form

purified from hybridoma cell culture

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

-

biological source

goat

biological source

goat

technique(s)

indirect ELISA: 1:10,000

technique(s)

-

technique(s)

quantitative precipitin assay: suitable

technique(s)

direct immunofluorescence: 1:16

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

FITC conjugate

isotype

IgG1

isotype

-

isotype

-

isotype

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters