KCQS03
KiCqStart® SYBR™ Green qPCR ReadyMix™
iQ™, with fluorescein for Bio-Rad systems
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
250 reactions | Comprobar disponibilidad del carrito | MXP 8,356.00 |
Acerca de este artículo
MXP 8,356.00
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5
detection method
SYBR™ Green
shipped in
dry ice
storage temp.
−20°C
General description
Es crucial una amplificación muy específica para conseguir una qPCR satisfactoria con tecnología de colorante SYBR Green I porque este colorante se une a, y detecta, cualquier dsDNA generado durante la amplificación. La ADN polimerasa Taq de inicio en caliente es inactivada por anticuerpo antes de la etapa inicial de desnaturalización de la PCR.
Application
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
- Expresión génica
- Cuantificación del ADN
- CHiP
Features and Benefits
- Resultados del análisis en tan sólo 33 minutos
- Resultados de la PCR en tiempo real muy eficaces y sensibles
- Se requiere poca o ninguna optimización
Other Notes
La mezcla KiCqStart SYBR Green qPCR ReadyMix es estable durante 1 año cuando se conserva en un refrigerador a una temperatura constante de -20°C, protegida de la luz. Para su conveniencia, puede conservarse sin congelar entre +2 y +8°C durante un máximo de 6 meses. Después de descongelar, mezcle bien antes de usar. No se recomienda congelar y descongelar repetidamente el producto. Sin embargo, se demostró que el producto no perdía rendimiento después de 20 ciclos de congelación-descongelación o 2 meses a +20°C.
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Legal Information
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Este artículo | |||
|---|---|---|---|
| description iQ™, with fluorescein for Bio-Rad systems | description For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems | description Low ROX™, for ABI and Stratagene instruments | description with ROX™ for ABI instruments |
| technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable |
| compatibility for use with Bio-Rad MyiQ, for use with Bio-Rad iQ 5, for use with Bio-Rad iCycler iQ | compatibility for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480 | compatibility for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000 | compatibility for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 HT Fast, for use with ABI StepOne, for use with ABI StepOnePlus |
| detection method SYBR™ Green | detection method SYBR™ Green | detection method SYBR™ Green | detection method SYBR™ Green |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| color colorless | color colorless | color colorless | color colorless |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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