KCQS03
KiCqStart® SYBR® Green qPCR ReadyMix™
iQ™, with fluorescein for Bio-Rad systems
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| Conditionnement | Référence | Disponibilité | Prix |
|---|---|---|---|
| 250 reactions | Vérifier la disponibilité des articles du panier | 338,00 € |
A propos de cet article
338,00 €
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5
detection method
SYBR® Green
shipped in
dry ice
storage temp.
−20°C
General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
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Cet article | |||
|---|---|---|---|
| technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) qPCR: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| compatibility for use with Bio-Rad MyiQ, for use with Bio-Rad iQ 5, for use with Bio-Rad iCycler iQ | compatibility for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480 | compatibility for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000 | compatibility for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus |
| feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| color colorless | color colorless | color colorless | color colorless |
| form liquid | form liquid | form liquid | form liquid |
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Notre protocole de qPCR avec SYBR Green est conçu pour quantifier précisément l'expression génique ou des réactions de RT-PCR.
Articles
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Contenu apparenté
La RT-qPCR, ou PCR quantitative après transcription inverse, combine les effets de la transcription inverse et de la PCR quantitative, ou PCR en temps réel, pour amplifier et détecter des cibles spécifiques. La RT-qPCR trouve de multiples applications, notamment dans la quantification du niveau d'expression génique, la validation de l'interférence par ARN ou la détection d'agents pathogènes comme les virus.
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| KCQS03-SAMPLE | 04061838099495 |
| KCQS03-250RXN | 04061833945117 |
| KCQS03-1250RXN | 04061833945100 |
| KCQS03-5000RXN | 04061833253533 |