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KCQS06

KiCqStart® Probe qPCR ReadyMix, ROX

with ROX for ABI instruments

Sinonimo/i:

Kicqstart® Probe qPCR ReadyMix

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250 reactions
Per conoscere la disponibilità, visualizza il carrello
CHF 215.00
1250 reactions
Per conoscere la disponibilità, visualizza il carrello
CHF 966.00

Informazioni su questo articolo

NACRES:
NA.55
UNSPSC Code:
41106300

CHF 215.00


Per conoscere la disponibilità, visualizza il carrello

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form

liquid

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included, hotstart

storage condition

protect from light

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

color

light blue

input

purified DNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7700, ABI 7900 HT Fast, ABI 7900 HT, ABI 7900, ABI StepOne, ABI StepOnePlus

detection method

probe-based

shipped in

dry ice

storage temp.

−20°C

General description

KiCqStart® Probe qPCR ReadyMix is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

KiCqStart® Probe qPCR ReadyMix, ROX has been used for amplification of cDNA of a stemness markers genes by quantitative reverse transcription-polymerase chain reaction (RT-qPCR).

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

Features and Benefits

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Other Notes

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

Legal Information

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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Questo articolo
KCQS05KCQS04KCQS09
description

with ROX for ABI instruments

description

with Low ROX for ABI and Stratagene instruments

description

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

description

with ROX for ABI instruments

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

light blue

color

light blue

color

light blue

color

light blue

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

usage

sufficient for 1250 reactions, sufficient for 5000 reactions, sufficient for 250 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

usage

sufficient for 500 reactions


Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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