KMM-101NV
KOD One™ PCR Master Mix
Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR
Synonym(s):
Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One™ PCR Ready Mix
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
0.25 mL | Check Cart for Availability | $68.40 |
5 mL | Check Cart for Availability | $489.00 |
About This Item
biological source
Escherichia coli (carrying the cloned UKOD DNA polymerase gene)
form
liquid
usage
sufficient for 25 reactions, sufficient for 500 reactions
feature
Fast PCR, High Fidelity PCR, dNTPs included, hotstart
technique(s)
PCR: suitable, whole genome amplification: suitable
input
crude DNA
application(s)
cannabis testing
shipped in
dry ice
storage temp.
−20°C
General description
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
Application
Features and Benefits
- Fast: Amplifies the targets using the following very short conditions:
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
- Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
- High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
- Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
- High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
Packaging
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR | description Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR | description ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR | description High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. |
| technique(s) PCR: suitable, whole genome amplification: suitable | technique(s) whole genome amplification: suitable, PCR: suitable | technique(s) whole genome amplification: suitable, PCR: suitable, PCR: suitable ((2X PCR Master Mix for high fidelity)) | technique(s) PCR: suitable, whole genome amplification: suitable |
| biological source Escherichia coli (carrying the cloned UKOD DNA polymerase gene) | biological source Escherichia coli (carrying the cloned UKOD DNA polymerase gene) | biological source - | biological source - |
| usage sufficient for 500 reactions, sufficient for 25 reactions | usage sufficient for 500 reactions, sufficient for 25 reactions | usage - | usage sufficient for 200 reactions, sufficient for 1000 reactions |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| form liquid | form liquid | form - | form - |
| application(s) cannabis testing | application(s) cannabis testing | application(s) - | application(s) - |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Storage Class
12 - Non Combustible Liquids
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