Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
L0881
Lectin from Arachis hypogaea (peanut)
lyophilized powder, Affinity-purified
Synonym(s):
PNA, Peanut agglutinin
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Available to ship TODAYfromMILWAUKEE | $135.00 |
10 mg | Available to ship TODAYfromMILWAUKEE | $260.00 |
25 mg | Available to ship TODAYfromMILWAUKEE | $515.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
form
lyophilized powder
Quality Segment
potency
<0.1 μg per mL agglutination activity
quality
Affinity-purified
impurities
salt, free
storage temp.
−20°C
General description
Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.
Application
Lectin from Arachis hypogaea (peanut) has been used:
- to detect Thomson-Friedenreich antigen
- as a component in PBS to study its effects on cell viability
- to study its ability to block the binding site
Biochem/physiol Actions
Lectin from Arachis hypogaea (peanut) possess specificity towards β -D-Gal(1-3)-D-galNAc residue of Thomson − Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, Affinity-purified | description FITC conjugate, lyophilized powder | description peroxidase conjugate, lyophilized powder | description Type II, lyophilized powder, ≥60 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities salt, free | impurities - | impurities - | impurities - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| potency <0.1 μg per mL agglutination activity | potency - | potency - | potency - |
| quality Affinity-purified | quality - | quality - | quality - |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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