M4655
Minimum Essential Medium Eagle
With Earle′s salts, L -glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
EMEM, MEM
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
500 mL | Available to ship TODAYfromMILWAUKEE | $38.90 |
1 L | Available to ship TODAYfromMILWAUKEE | $62.80 |
6 x 500 mL | Check Cart for Availability | $203.00 |
24 x 500 mL | Check Cart for Availability | $731.00 |
About This Item
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’ or Earle’s salts has broadened the usefulness of this medium.
Application
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description With Earle′s salts, | description Modified, with Earle′s salts and reduced NaHCO3. without | description With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With Earle′s salts and sodium bicarbonate, without |
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components sodium pyruvate: no | components phenol red: 0.011 g/L | components glucose: 1.0 g/L (Dextro) | components phenol red: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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