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α-Mannosidase from Canavalia ensiformis (Jack bean)

Name: α-Mannosidase from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA
Price: 183 USD
Availability: InStock

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

Synonym(s):

α-D-Mannoside mannohydrolase

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Size/SKU	Availability	Price
1 mg	Check Cart for Availability	$183.00
5 mg	Check Cart for Availability	$641.00
25 mg	Check Cart for Availability	$2,460.00 $2,091.00

About This Item

CAS Number:

9025-42-7

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-794-1

MDL number:

MFCD00130495

EC Number:

3.2.1.24 (BRENDA, IUBMB)

Specific activity:

≥15 units/mg protein (biuret)

Biological source:

Canavalia ensiformis

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Properties

biological source

Canavalia ensiformis

Quality Segment

200

form

ammonium sulfate suspension

specific activity

≥15 units/mg protein (biuret)

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

storage temp.

2-8°C

SMILES string

[Zn+2]

InChI

1S/Zn/q+2

InChI key

PTFCDOFLOPIGGS-UHFFFAOYSA-N

Description

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

Liberates mannose from a variety of synthetic and natural α-mannosides.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

for the digestion of neutral glycans.
to remove O-glycans from recombinant β-glucosidase
for the inhibition of renal fibroblasts proliferation

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

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This Item	A2264	C7642	U1500
Sigma-Aldrich M7257 α-Mannosidase from Canavalia ensiformis (Jack bean)	Sigma-Aldrich A2264 β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)	Sigma-Aldrich C7642 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U1500 Urease from Canavalia ensiformis (Jack bean)
description ammonium sulfate suspension, ≥15 units/mg protein (biuret)	description ammonium sulfate suspension, ≥8 units/mg protein	description FITC conjugate, Type IV, lyophilized powder	description Type III, powder, 15,000-50,000 units/g solid
biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis
specific activity ≥15 units/mg protein (biuret)	specific activity ≥8 units/mg protein	specific activity -	specific activity 15,000-50,000 units/g solid
form ammonium sulfate suspension	form ammonium sulfate suspension	form lyophilized powder	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
foreign activity β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%	foreign activity α-mannosidase ≤0.5%, α- and β-galactosidase, and α-L-fucosidase ≤0.1%	foreign activity -	foreign activity -

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

2 years ago

α-マンノシダーゼ Canavalia ensiformis（タチナタマメ）由来　1mg　を以前購入しましたが、何マイクロリットルの何の溶液に溶解しるか教えてください。

1 answer
1. Technical Support
  
  2 years ago
  
  This product is a 1 – 10 mg/mL suspension in 3.0 M (NH4)2SO4 containing 0.1 mM zinc acetate. The volume varies from lot to lot and is reported in the product Certificate of Analysis. For example, a lot reporting a concentration of 5 mg protein/ml will have a volume of 200 uL for the 1 mg package size. Please see the link below to review a sample of lot specific Certificate:
  https://www.sigmaaldrich.com/product/sigma/m7257#product-documentation
  Please see the following link to review additional information available in the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/217/622/m7257pis.pdf
  
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