NA2120
GenElute™ Bacterial Genomic DNA Kits
sufficient for 350 purifications
Synonym(s):
Bacterial Genomic DNA, bacterial DNA prep, Gen Elute
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship TODAYfromMILWAUKEE | $1,010.00 |
About This Item
$1,010.00
Available to ship TODAYDetails
usage
sufficient for 350 purifications
Quality Segment
technique(s)
DNA purification: suitable
test parameters
: 70—120 min kit run time, sample volume: 1.5 mL bacterial culture
storage temp.
15-25°C
General description
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
GenElute™ Bacterial Genomic DNA Kits has been used:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
Biochem/physiol Actions
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Features and Benefits
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
- Typical DNA yields of 15 μg - 20 μg
- Protocols provided for Gram + and Gram - bacteria
- High quality genomic DNA in less than 2 hours
- Purified DNA has an A260/A280 ratio between 1.6 and 1.9
Other Notes
Legal Information
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This Item | |||
|---|---|---|---|
| description sufficient for 350 purifications | description sufficient for 70 purifications | description sufficient for 10 purifications | description sufficient for 70 purifications |
| usage sufficient for 350 purifications | usage sufficient for 70 purifications | usage sufficient for 10 purifications | usage sufficient for 70 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| test parameters sample volume: 1.5 mL bacterial culture, : 70—120 min kit run time | test parameters : 70—120 min kit run time, sample volume: 1.5 mL bacterial culture | test parameters : 70—120 min kit run time, sample volume: 1.5 mL bacterial culture | test parameters : <40 min kit run time, sample volume: 200 μL |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
NA2100 | NA2120, NA2100 | NA2110, NA2120 | BLD9601, NA2020, BLD9604 |
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Signal Word
Danger
Target Organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
Flash Point (°F)
77.0 °F - closed cup
Flash Point (°C)
25 °C - closed cup
WGK
WGK 3
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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