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Phosphatase, Alkaline from bovine intestinal mucosa

Name: Phosphatase, Alkaline from bovine intestinal mucosa
Brand: SIGMA
Price: 636 USD
Availability: InStock

BioUltra, ≥5,700 DEA units/mg protein

Synonym(s):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Size/SKU	Availability	Price
10000 units	Estimated to ship TODAYfromSAINT LOUIS	$636.00
50000 units	Check Cart for Availability	$2,400.00

About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

eCl@ss:

42010105

NACRES:

NA.54

EC Number:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

Specific activity:

≥5,700 DEA units/mg protein

Concentration:

≥10 mg/mL

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Properties

product line

BioUltra

Quality Segment

200

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

specific activity

≥5,700 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

technique(s)

protein detection: suitable

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl₂and 0.12 mM ZnCl₂, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

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This Item	A2356	P7923	P7640
Sigma-Aldrich P0114 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich A2356 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P7923 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P7640 Phosphatase, Alkaline from bovine intestinal mucosa
description BioUltra, ≥5,700 DEA units/mg protein	description ≥5,500 DEA units/mg protein	description buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein	description lyophilized powder, ≥10 DEA units/mg solid
technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable
specific activity ≥5,700 DEA units/mg protein	specific activity ≥5,500 DEA units/mg protein	specific activity ≥4,000 DEA units/mg protein	specific activity ≥10 DEA units/mg solid
form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)	form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)	form buffered aqueous glycerol solution	form lyophilized powder
concentration ≥10 mg/mL	concentration ≥10 mg/mL	concentration ≥10.0 mg/mL	concentration -
shipped in wet ice	shipped in -	shipped in -	shipped in wet ice
mol wt dimer ~160 kDa	mol wt dimer ~160 kDa	mol wt dimer ~160 kDa	mol wt dimer ~160 kDa

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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