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P8361

Phosphatase, Alkaline bovine

recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Size/SKUAvailabilityPrice
5000 units

Estimated to ship onOctober 06, 2026fromMILWAUKEE

$596.00

About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
MFCD00131849
EC Number:
3.1.3.1 (BRENDA, IUBMB)
eCl@ss:
42010105
Specific activity:
≥4000 units/mg protein
Recombinant:
expressed in Pichia pastoris

$596.00

Estimated to ship onOctober 06, 2026Details

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recombinant

expressed in Pichia pastoris

Quality Segment

form

solid

specific activity

≥4000 units/mg protein

mol wt

apparent mol wt 160 kDa

technique(s)

protein detection: suitable

storage temp.

2-8°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. Product P8361 has been used to dephosphorylate Dishevelled (Dvl) , which is a key component in the Wnt/β-catenin signaling pathway.
An assay, using this ALP enzyme, was developed to assess the cryoprotective activity of two proteins CRP-1 and CRP-2. The enzyme has been used for inhibitory studies of cyclic nucleotide analogs that inhibit prostatic acid phosphatase using a fluorogenic assay. It is commonly used as a "reporter" in detection systems, in which it is conjugated to a protein (antibody, streptavidin, etc.) that specifically recognizes a target molecule. Alkaline phosphatase has also been used to dephosphorylate casein.
The product is manufactured without the use of any animal source materials during fermentation or purification.

Biochem/physiol Actions

Alkaline phosphatase is a dimer consisting of two equal subunits. The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges. Maximal activity with alkaline phosphatase is achieved in the presence of magnesium. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate. Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase. The enzyme is most stable in the pH range 7.5-9.5.2. The pH optimum for enzymatic activity is pH 8-10.
Alkaline phosphatase dephosphorylates proteins and nucleic acids.

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

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Phosphatase, Alkaline bovine recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

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P6774

Phosphatase, Alkaline from bovine intestinal mucosa

description

recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

description

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

description

≥2,000 DEA units/mg protein

description

buffered aqueous solution, ≥2,000 DEA units/mg protein

specific activity

≥4000 units/mg protein

specific activity

-

specific activity

≥2,000 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

recombinant

expressed in Pichia pastoris

recombinant

expressed in proprietary host

recombinant

-

recombinant

-

form

solid

form

buffered aqueous glycerol solution

form

aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

apparent mol wt 160 kDa

mol wt

-

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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