Skip to Content
MilliporeSigma

Skip To

R8755

RPMI-1640 Medium

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
10 L

Available to ship TODAYfromMILWAUKEE

$78.20
10 x 1 L

Available to ship TODAYfromMILWAUKEE

$80.30

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:

$78.20


Available to ship TODAYDetails


Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

RPMI-1640 Medium, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium is used for the following applications:
  • Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
  • Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • Used for cell isolation and culture
  • Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
  • Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • Used for the preparation of antifungal agents

Preparation Note

Formulated to contain 10.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
R7755R0883R7638
description

Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

description

AutoMod, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

description

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

liquid

form

liquid

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells

classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells


Still not finding the right product?

Explore all of our products under RPMI-1640 Medium


Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

Reviews

No rating value

Active Filters