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S1629

Sulfatase from Aerobacter aerogenes

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Size/SKUAvailabilityPrice
10 units

Available to ship TODAYfromMILWAUKEE

$137.00
50 units

Available to ship TODAYfromMILWAUKEE

$444.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL

$137.00


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type

Type VI

Quality Segment

form

buffered aqueous glycerol solution

specific activity

2-5 units/mg protein (biuret)

mol wt

~41 kDa

concentration

10-20 units/mL

foreign activity

β-Glucuronidase ≤10 U/mL

shipped in

wet ice

storage temp.

−20°C

General description

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Application

Sulfatase from Aerobacter aerogenes has been used:
  • as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
  • in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
  • to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Biochem/physiol Actions

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

Physical form

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.

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This Item
S9751S9626S9754
description

Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL

description

Type H-2, aqueous solution, ≥2,000 units/mL

description

Type H-1, sulfatase ≥10,000 units/g solid

description

Type VIII, lyophilized powder, 20-40 units/mg solid

specific activity

2-5 units/mg protein (biuret)

specific activity

-

specific activity

-

specific activity

20-40 units/mg solid

concentration

10-20 units/mL

concentration

≥2,000 units/mL

concentration

-

concentration

-

form

buffered aqueous glycerol solution

form

aqueous solution

form

powder

form

lyophilized powder

mol wt

~41 kDa

mol wt

~85 kDa by gel filtration

mol wt

~85 kDa by gel filtration

mol wt

~43 kDa by gel filtration

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-


Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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