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S5171

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Synonym(s):

sFN

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Size/SKUAvailabilityPrice
0.5 mg

Available to ship TODAYfromMILWAUKEE

$530.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00284706
Biological source:
human
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Mol wt:
~235-270 kDa

$530.00

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Product Name

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

biological source

human

Quality Segment

recombinant

expressed in E. coli

product line

BioReagent

form

solution

mol wt

~235-270 kDa

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1 μg/cm2

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

Application

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Preparation Note

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Other Notes

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Disclaimer

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

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Superfibronectin from human plasma solution, BioReagent, suitable for cell culture

Sigma-Aldrich

S5171

Superfibronectin from human plasma

FIBRONECTIN FROM HUMAN PLASMA liquid, 0.1% (Solution), BioReagent, suitable for cell culture

Sigma-Aldrich

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FIBRONECTIN FROM HUMAN PLASMA

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

Sigma-Aldrich

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Fibronectin human plasma

Fibronectin human plasma lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F2006

Fibronectin human plasma

description

solution, BioReagent, suitable for cell culture

description

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

description

lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

description

lyophilized powder, BioReagent, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

human

biological source

human plasma

biological source

human plasma

biological source

human plasma

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mol wt

~235-270 kDa

mol wt

-

mol wt

-

mol wt

450 kDa

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

solution

form

liquid

form

lyophilized powder

form

lyophilized powder

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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