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Stem Cell Factor human

Name: Stem Cell Factor human
Brand: SIGMA
Price: 624 CAD

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable

Synonym(s):

SCF, c-Kit ligand

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Size/SKU	Availability	Price
10 μg	Please contact Customer Service for Availability	$624.00
50 μg	Please contact Customer Service for Availability	$929.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00165757

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥95% (SDS-PAGE)

Form:

powder

Mol wt:

monomer 18.6 kDa ((165 aa))

Impurities:

≤1 EU/μgtested (LAL test)

$624.00

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Properties

Product Name

Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

powder

potency

≤15 ng/mL ED₅₀

quality

endotoxin tested

mol wt

monomer 18.6 kDa ((165 aa))

packaging

pkg of 10 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μgtested (LAL test)

UniProt accession no.

P21583

storage temp.

−20°C

Description

Application

Stem Cell Factor human has been used in

In vitro differentiation of equine embryonic stem cells[1]
Chemotaxis detection system[2]
Ex vivo expansion of umbilical cord blood CD34⁺ cells
Cytokine therapy

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine.[3] SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.[3]

Physical form

Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

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This Item	H8416	S9915	T7799
Sigma-Aldrich S7901 Stem Cell Factor human	Sigma-Aldrich H8416 Stem Cell Factor human	Sigma-Aldrich S9915 Stem Cell Factor from mouse	Sigma-Aldrich T7799 Tumor Necrosis Factor-β human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE and HPLC)	assay >95% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli	recombinant expressed in E. coli
biological source human	biological source human	biological source mouse	biological source human
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form lyophilized powder	form powder	form powder

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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