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SAE0075

Angiotensin Converting Enzyme, ACE, human

recombinant, ≥10 U/mg, expressed in HEK 293 cells

Synonym(s):

ACE, ACE1, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase

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Size/SKUAvailabilityPrice
0.5 units
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$339.00

About This Item

EC Number:
3.4.15.1 (BRENDA, IUBMB)
NACRES:
NA.77
UNSPSC Code:
12352200
Form:
lyophilized powder
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

$339.00

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recombinant

expressed in HEK 293 cells

Quality Segment

assay

≥95% (SDS-PAGE)

form

lyophilized powder

specific activity

≥10 U/mg

mol wt

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23.

Biochem/physiol Actions

The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU
Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Other Notes

One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC.

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Angiotensin Converting Enzyme, ACE, human recombinant, ≥10 U/mg, expressed in HEK 293 cells

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Sigma-Aldrich

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Angiotensin Converting Enzyme from rabbit lung ≥2.0 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

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Angiotensin Converting Enzyme from rabbit lung

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 clone 9B9, Chemicon®, from mouse

Sigma-Aldrich

MAB4051

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9

description

recombinant, ≥10 U/mg, expressed in HEK 293 cells

description

biotin-tagged, human recombinant, ≥1000000U/mg, expressed in HEK 293 cells

description

≥2.0 units/mg protein (modified Warburg-Christian)

description

clone 9B9, Chemicon®, from mouse

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

-

recombinant

-

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

-

assay

-

form

lyophilized powder

form

-

form

lyophilized powder

form

-

mol wt

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

mol wt

-

mol wt

-

mol wt

-

UniProt accession no.

P12821

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P12821

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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