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Sigma 7-9^®

Name: Sigma 7-9<SUP>&#174;</SUP>
Brand: SIGMA
Price: 37.2 USD
Availability: InStock

≥99% (titration), crystalline, Sigma 7-9^®

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Synonym(s):

Trizma^® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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100 g	Available to ship TODAY	$37.20
500 g	Available to ship TODAY	$88.80
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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

NACRES:

NA.25

PubChem Substance ID:

24899961

UNSPSC Code:

12352104

EC Number:

201-064-4

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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Properties

Product Name

Sigma 7-9^®, ≥99% (titration), powder or crystals

description

aminopeptidase substrate

Quality Segment

200

assay

≥99% (titration)

form

powder or crystals

storage condition

dry at room temperature

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable

impurities

≤0.5% water (Karl Fischer)

color

white to off-white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 0.667 g/mL, clear, colorless to faintly yellow

absorption

≤0.4 at 290 at 40%

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Sigma 7-9^® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9^® has been used:

as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits

Suitable for Biological and Biochemical Research
Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

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This Item	T6066	93350	T1699
Sigma-Aldrich T1378 Sigma 7-9^®	Sigma-Aldrich T6066 Trizma^® base	Sigma-Aldrich 93350 Trizma^® base	Sigma-Aldrich T1699 Trizma^® base solution
description ≥99% (titration), powder or crystals	description BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)	description 99.7% (T), puriss. p.a.	description 1.5 M
assay ≥99% (titration)	assay ≥99.9% (titration)	assay 99.7% (T)	assay -
technique(s) flow cytometry: suitable, ELISA: suitable, immunohistochemistry: suitable	technique(s) protein purification: suitable, cell culture \| mammalian: suitable, DNA extraction: suitable	technique(s) -	technique(s) -
Quality Level 200	Quality Level 400	Quality Level 200	Quality Level 200
application(s) clinical research diagnostic assay manufacturing life science and biopharma sample preparation	application(s) clinical research clinical testing diagnostic assay manufacturing	application(s) -	application(s) diagnostic assay manufacturing
form powder or crystals	form crystalline powder	form crystalline	form liquid
storage condition dry at room temperature	storage condition dry at room temperature	storage condition -	storage condition -

Storage Class

11 - Combustible Solids

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

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