Biological Buffers
It’s important to protect the integrity of your biomolecules and reagents by choosing the right biological buffer systems. We offer an extensive portfolio of biological buffers for a wide variety of applications, including cell culture buffers, PCR buffers, HEPES buffers, and assay buffers.
Key features of our advanced buffer systems:
- High-purity solutions available in a wide range of grades
- Multiple and user-friendly packaging options
- Assured solution stability with pH control
- No interference with biological processes
- Add critical salts and nutrients to cells and tissues
- Scalable from early research to commercial applications
- Customized blending
Dulbecco′s Phosphate Buffered Saline
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Phosphate buffered saline
tablet, pH 7.2-7.6 (1 tablet/200 mL)
Trizma® base
≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate
HEPES
≥99.5% (titration)
Phosphate buffered saline
powder, pH 7.4, for preparing 1 L solutions
MES hydrate
≥99.5% (titration)
HEPES
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
HEPES solution
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
Sodium bicarbonate
powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture
Triethylammonium acetate buffer
volatile buffer, ~1.0 M in H2O
RIPA Buffer
Trizma® base
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
Trizma® hydrochloride
reagent grade, ≥99.0% (titration), crystalline
MOPS
≥99.5% (titration), crystalline powder
Ethylenediaminetetraacetic acid disodium salt dihydrate
suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)
Imidazole
ReagentPlus®, 99%
Dulbecco′s Phosphate Buffered Saline
Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Dulbecco′s Phosphate Buffered Saline
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
MES hydrate
BioPerformance Certified, suitable for cell culture, ≥99.5%
Potassium phosphate monobasic
powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Our advanced buffering systems can bring phenomenal stability to your cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation-based applications. All our products can be scaled from early research to commercial applications.
Related Resources
- Buffer Reference Center
These tables will help you navigate the preparation of many common buffer solutions by pH and pKa. Choose a buffer with an effective pH range for your specific assay.
- Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
- TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
- M-Clarity™ Program
The MQ levels of the M-Clarity™ Program provide transparency so that you can choose, with confidence, suitable products that meet your needs.
- Buffer Calculator
Buffer solution calculator: Empirical formula, pKa, and buffer pH range calculations for various buffers.
- Poster: Biological Buffers Selection Guide
- Flyer: pHast Pack Electrophoresis
The pHast Pack™ platform was developed to expedite your research and provide a convenient solution for tedious lab tasks.
- Flyer: Tricine Buffer
Our convenient Tricine high purity biological buffer was developed for unparalleled quality and economy.
- Flyer: HEPES Buffer
Our high-purity biological buffers will be HEPES of help for your research.
- Flyer: Trizma Buffers
Our convenient high purity Trizma® (tris) hydrochloride buffer and basimetric primary standard was developed for impeccable quality at a budget price.
- Brochure: Biological Buffers
High-purity biological buffers from bench to bulk volumes.
- pHast Pack™ – Ready-to-Use Buffers
Simplify your buffer solution preparation with our pHast Pack™ ready-to-use buffers which offer exact concentrations and pH without weighing, buffer calculations, and pH adjustments for reliable research results.
Related Webinars
Complete Workflow Solutions
We provide a comprehensive selection of high-quality biochemicals in a wide range of buffer grades and innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing and customized blending.
Bioperformance Certified Buffers
Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocessing workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include enzymatic assay of nuclease, protease, and phosphatase, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures the reproducibility of your experiment.
pHast Pack™ Ready-to-Use Buffers
Trying to get the correct concentration and pH value in buffer preparations can be a tedious and time-consuming task. Our pHast Pack™ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick and reproducible results again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.
Need help making a buffer? Our buffer preparation calculator helps you to quickly calculate buffer molarity, mass, and volume.
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