Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

XNATRG

SYBR™ Green Extract-N-Amp™ Tissue PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Synonym(s):

Tissue direct PCR kit, Tissue direct real-time PCR

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 kit
Check Cart for Availability
$3,560.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41106303

$3,560.00

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Segment

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

feature

dNTPs included, hotstart, Direct PCR

technique(s)

PCR: suitable, whole genome amplification: suitable

input

crude DNA

storage temp.

−20°C

General description

The SYBR™ Green Extract-N-Amp™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Application

SYBR™ Green Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and genotyping.

Biochem/physiol Actions

DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp™ SYBR™ Green PCR ReadyMix™ and user-provided PCR primers.

Features and Benefits

  • Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
  • Fast – tissue to qPCR in 15 minutes
  • Convenient – no long enzymatic digestions and no column purifications
  • Simple – rapid, easy-to-follow protocol
  • Sensitive – specially formulated HOTSTART® SYBR™ Green PCR ReadyMix™ for highly specific PCR amplification and quantitation of genomic DNA
  • Safe – no organic extraction with hazardous chemicals

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Kit contains Extraction Solution, Neutralization Solution, Tissue Preparation Solution, and Extract-N-Amp SYBR Green PCR ReadyMix™. The Extract-N-Amp SYBR Green PCR ReadyMix is a 2X reaction mix containing SYBR Green, buffer, salts, dNTPs, Taq polymerase and JumpStart™ Taq antibody. It is optimized specifically for use with the extraction reagents and contains JumpStart Taq antibody for hot start PCR to enhance specificity and SYBR Green I to act as a nonspecific reporter for real-time PCR.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
XNATGXNAT2XNAPG
SYBR™ Green Extract-N-Amp™ Tissue PCR Kit sufficient for 1000 extractions, sufficient for 1000 amplifications

Sigma-Aldrich

XNATRG

SYBR™ Green Extract-N-Amp™ Tissue PCR Kit

SYBR™ Green Extract-N-Amp™ Tissue PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

Sigma-Aldrich

XNATG

SYBR™ Green Extract-N-Amp™ Tissue PCR Kit

Extract-N-Amp™ Tissue PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

Sigma-Aldrich

XNAT2

Extract-N-Amp™ Tissue PCR Kit

SYBR™ Green Extract-N-Amp™ Plant PCR Kit sufficient for 100 preparations

Sigma-Aldrich

XNAPG

SYBR™ Green Extract-N-Amp™ Plant PCR Kit

description

sufficient for 1000 extractions, sufficient for 1000 amplifications

description

sufficient for 100 extractions, sufficient for 100 amplifications

description

sufficient for 100 extractions, sufficient for 100 amplifications

description

sufficient for 100 preparations

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

whole genome amplification: suitable, RT-qPCR: suitable

input

crude DNA

input

crude DNA

input

crude DNA

input

crude DNA

usage

sufficient for 1000 reactions, sufficient for 1000 amplifications, sufficient for 1000 extractions

usage

sufficient for 100 extractions, sufficient for 100 reactions, sufficient for 100 amplifications

usage

sufficient for 100 reactions, sufficient for 100 amplifications, sufficient for 100 extractions

usage

sufficient for 100 preparations, sufficient for 100 reactions

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

feature

Direct PCR, hotstart, dNTPs included

feature

dNTPs included, hotstart, Direct PCR

feature

Direct PCR, dNTPs included, hotstart

feature

dNTPs included, hotstart

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Still not finding the right product?

Explore all of our products under SYBR® Green Extract-N-Amp™ Tissue PCR Kit

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class

10 - Combustible liquids

WGK

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters