No protocol is available for labeling His6-tagged protein with this product. Product 76508 is an Atto 647N Protein Labeling Kit, which includes a protocol in the documentation section.
02175
NTA - Atto 647 N
BioReagent, suitable for fluorescence, ≥80% (HPCE)
Synonym(s):
Atto 647 N - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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| Size/SKU | Availability | Price |
|---|---|---|
250 μg | Available to ship TODAYfromMILWAUKEE | $432.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12171501
product line
BioReagent
Quality Segment
assay
≥80% (HPCE)
fluorescence
λex 647 nm; λem 669 nm in PBS
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
General description
find more information here
Application
for the fluorescence detection of HIS-tagged proteins
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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This Item | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, ≥80% (HPCE) | description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (HPLC) |
| fluorescence λex 647 nm; λem 669 nm in PBS | fluorescence λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide | fluorescence λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide | fluorescence - |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| assay ≥80% (HPCE) | assay ≥80% (sum of isomers, HPCE) | assay ≥80% (sum of isomers, HPCE) | assay ≥80% (HPLC) |
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Do you have any protocol to label NTA - Atto 647 N to His6-Taggged protein?
1 answer-
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Is this NTA-Atto 647N product supplied with Ni2+ included? I'm wondering that I should have to treat additional Ni2+ for NTA-Atto 647N function.
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This product is already complexed with nickel.
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Can this NTA-atto 647N be applied to display the live cell covered with his-tagged protein?
1 answer-
There is no protocol available for using this product with live cells.
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Hi! What is the recommendations for storing of dissolved in PBS Ni-NTA-atto-647? Can I do multiple freeze/thaw cycles?
1 answer-
The solution stability of NTA - Atto 647 N has not been evaluated. The original Atto 647 N is not stable in aqueous solutions for long-term storage and should be prepared fresh. Solutions prepared in anhydrous, amine-free DMSO or Dimethylformamide may offer some benefit to solution stability. It would be up to the end user to determine long-term storage, however, general practice is typically to aliquot and store at -20 °C, avoiding repeated freeze/thaw cycles.
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What is the molecular weight of this product ?
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The molecular weight of this product is 1120 g/mol.
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How is Product 02175, NTA-Atto 647N, to be dissolved and diluted?
1 answer-
The 250 μg of 02175-250UG-F, NTA-Atto 647N, may be dissolved in 250 μL of phosphate buffered saline (PBS), pH 7.4, to make a 1 mg/mL stock solution. This stock solution may be diluted by a factor of 1:1000 as follows:---For use on gel: 25 μL of stock in 25 mL PBS.---For use on Western Blot (PVDF membrane): 25 μL of stock in 25 mL PBST (PBS plus 0.1% TWEEN 20).
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