Chromolith® HPLC and UHPLC Columns

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology. There are no silica particles.

Several key benefits result directly from the bimodal pore structure of the silica gel, which has 2 µm macropores and 13 nm mesopores:

  • Rapid separations at very low column back-pressure
  • Standard HPLC instruments are fully compatible with all Chromolith® columns and UHPLC instruments are fully compatible with Chromolith® UHPLC 2mm i.d. columns.
  • Matrix-rich samples (such as food or life science samples) can be analyzed without the need for sophisticated and time-consuming sample preparation. Guard column cartridges are also available.
  • Cost-savings are achieved as the column lifetimes are much longer than for particulate HPLC columns, in particular when analyzing matrix-rich samples.
  • Complex multi-component samples can be separated either by using Chromolith® HighResolution (HR) columns or by using long very high-efficiency columns formed by connecting two or more Chromolith® columns together. The low column backpressure makes this possible.
  • Easy transfer of methods from a particulate column to a Chromolith® column.

The columns are available not only with octadecyl (RP-18e) or octyl (RP-8e) endcapped surface modification, but also with Phenyl, CN (nitrile), Diol and NH2 (amino) surface modification as well as an unmodified pure silica.

 

  130 Å for Chromolith® columns 150 Å for Chromolith® HighResolution columns
  Macropore: 2 µm
Mesopore: 130 Å
Macropore: 1.15 µm
Mesopore: 150 Å 
Chromolith® HighResolution RP-18e  
Chromolith® HighResolution RP-8e  
Chromolith® RP-18e  
Chromolith® RP-8e  
Chromolith® Phenyl
 
Chromolith® CN  
Chromolith® Diol  
Chromolith® NH2  
Chromolith® Si  

Chromolith® 2 mm ID: 1.5 µm

Chromolith® Prep 25 mm ID 3 µm Macropores

 

Specifications
Silica type High-purity
Base Material Monolithic
Macropore size 2 µm (Chromolith® HPLC columns)
1.5 µm (Chromolith® UHPLC columns)
1.15 µm (Chromolith® HighResolution columns)
Mesopore size 13 nm (130 Å for Chromolith® columns)
15 nm (150 Å for Chromolith®HighResolution columns)
Pore volume 1 mL/g
Total pore volume 3.2 mL/g (Chromolith® HPLC columns)
2.9 mL/g (Chromolith® HighResolution columns)
Total porosity >80 %
Surface area 300 m2/g (Chromolith® HPLC columns)
250 m2/g (Chromolith® HighResolution columns
Surface modification (USP classification) RP-18 – octadecyl endcapped - USP classification L1
RP-8 – octyl endcapped - USP classification L7
Phenyl - USP classification L11
CN (nitrile) - USP classification L10
DIOL - USP classification L20
NH2 (amine) - USP classification L8
Si (silica unmodified) - USP classification L3
Carbon content RP-18 – 18 %
RP-8 – 11 %
Phenyl
CN (nitrile)
DIOL
NH2 (amine)

 

The internal diameters of the analytical columns are 2 mm, 3 mm, 4.6 mm and guard column cartridges with holders are also available. Chromolith® SemiPrep and Prep columns (10 mm and 25 mm i.d.) are also available as well as Chromolith® CapRod® capillary columns.

The mesopores of the silica gel in all Chromolith® columns are 13 nm, which gives a high specific surface area of 300 m2/g and good retention of the analytes.

Chromolith®UHPLC columns are Chromolith® columns with 2 mm internal diameter. These columns are ideal for use with UHPLC or UPLC instruments, thanks to their very small internal volumes. A particular benefit is again the very fast analysis, reduced sample-preparation and the low column backpressure.

Electron-Microscope photo of monolithic silica structure

Electron-Microscope photo
of monolithic silica structure.

The silica itself is high purity, type B silica for inertness, reproducibility and stability, with modern bonding processes that optimize bonded phase coverage and maximize stability, while minimizing bleed and unwanted secondary interactions.

Chromolith® HighResolution (HR) columns are also available with the benefit of increased efficiency, very good peak symmetry and low column backpressure.

These have smaller 1.15 µm macropores, which significantly increase the separation efficiency (theoretical plate number) of the columns. The efficiency is approximately equivalent to 2.6 µm particulate columns whereas the backpressure is approximately equivalent to 5 µm columns.

 

Chromolith® HPLC and UHPLC monolithic silica columns

 

Chromolith® HPLC and UHPLC monolithic silica columns are cladded in inert PEEK (polyetheretherketone) polymeric material and can be connected directly to HPLC, UHPLC or UPLC system as a “ready to use” column.

Chromolith® HPLC and UHPLC monolithic silica columns

Materials

Product Modification I.d. Length Content Ordering No.
Chromolith® Validation Kit RP-18e 2 mm 100 mm 3 columns from 3
different batches
1.52062
Chromolith® Performance RP-18e 2 mm 100 mm 1 UHPLC/HPLC column 1.52006
Chromolith® FastGradient RP-18e 2 mm 50 mm 1 UHPLC/HPLC column 1.52007
Chromolith® Flash RP-18e 2 mm 25 mm 1 UHPLC/HPLC column 1.52014
Chromolith® Guard Cartridge Kit RP-18e 2 mm 5 mm 3 guard cartridges,
1 cartridge holder
1.52008
Chromolith® Guard Cartridge RP-18e 2 mm 5 mm 3 guard cartridges 1.52009
Chromolith® Validation Kit RP-18e 3 mm 100 mm 3 columns from 3
different batches
1.52063
Chromolith® Performance RP-18e 3 mm 100 mm 1 HPLC column 1.52001
Chromolith® FastGradient RP-18e 3 mm 50 mm 1 HPLC column 1.52002
Chromolith® Flash RP-18e 3 mm 25 mm 1 HPLC column 1.52003
Chromolith® Guard Cartridge Kit RP-18e 3 mm 5 mm 3 guard cartridges,
1 cartridge holder
1.52004
Chromolith® Guard Cartridge RP-18e 3 mm 5 mm 3 guard cartridges 1.52005
Chromolith® Performance RP-18e 4.6 mm 100 mm 1 HPLC column 1.02129
Chromolith® Validation Kit RP-18e 4.6 mm 100 mm 3 columns from 3
different batches
1.51466
Chromolith® SpeedROD RP-18e 4.6 mm 50 mm 1 HPLC column 1.51450
Chromolith® Flash RP-18e 4.6 mm 25 mm 1 HPLC column 1.51463
Chromolith® Guard Cartridge RP-18e 4.6 mm 10 mm 3 guard cartridges 1.51452
Chromolith® Guard Cartridge RP-18e 4.6 mm 5 mm 3 guard cartridges 1.51451
Chromolith® Validation Kit RP-18e 4.6 mm 100 mm 3 columns from 3
different batches
1.52019
Chromolith® HighResolution RP-18e 4.6 mm 150 mm 1 HPLC column 1.52023
Chromolith® HighResolution RP-18e 4.6 mm 100 mm 1 HPLC column 1.52022
Chromolith® HighResolution RP-18e 4.6 mm 50 mm 1 HPLC column 1.52021
Chromolith® HighResolution RP-18e 4.6 mm 25 mm 1 HPLC column 1.52020
Chromolith® HR Guard Cartridge RP-18e 4.6 mm 5 mm 3 guard cartridges 1.52025
Chromolith® Performance RP-8e 4.6 mm 100 mm 1 HPLC column 1.51468
Chromolith® HighResolution RP-8e 4.6 mm 100 mm 1 HPLC column 1.52064
Chromolith® Guard Cartridge RP-8e 4.6 mm 5 mm 3 guard cartridges 1.52013
Chromolith® Phenyl 4.6 mm 25 mm 1 HPLC column 1.52056
Chromolith® Phenyl 4.6 mm 50 mm 1 HPLC column 1.52057
Chromolith® Phenyl 4.6 mm 100 mm 1 HPLC column 1.52058
Chromolith® Guard Cartridge Phenyl 4.6 mm 5 mm 3 guard cartridges 1.52059
Chromolith® CN 4.6 mm 25 mm 1 HPLC column 1.52046
Chromolith® CN 4.6 mm 50 mm 1 HPLC column 1.52047
Chromolith® CN 4.6 mm 100 mm 1 HPLC column 1.52048
Chromolith® Guard Cartridge CN 4.6 mm 5 mm 3 guard cartridges 1.52050
Chromolith® DIOL 4.6 mm 25 mm 1 HPLC column 1.53170
Chromolith® DIOL 4.6 mm 50 mm 1 HPLC column 1.53171
Chromolith® DIOL 4.6 mm 100 mm 1 HPLC column 1.53172
Chromolith® Guard Cartridge DIOL 4.6 mm 5 mm 3 guard cartridges 1.53175
Chromolith® Performance Si 4.6 mm 100 mm 1 HPLC column 1.51465
Chromolith® Guard Cartridge Si 4.6 mm 5 mm 3 guard cartridges 1.52011
Chromolith® Performance NH2 4.6 mm 100 mm 1 HPLC column 1.52028
Chromolith® SpeedROD NH2 4.6 mm 50 mm 1 HPLC column 1.52027
Chromolith® Flash NH2 4.6 mm 25 mm 1 HPLC column 1.52026
Chromolith® Guard Cartridge NH2 4.6 mm 5 mm 3 guard cartridges 1.52030
Chromolith® Guard
Cartridge Holder
- 4.6 mm 5 mm 1 cartridge holder 1.52032
Chromolith® Guard
Cartridge Holder
- 4.6 mm 10 mm 1 cartridge holder 1.52033
Chromolith® Column Coupler -     1 column coupler 1.51467