MAB1083

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

• Sinônimo(s): Green Fluorescent protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 3F8.2, monoclonal
• reatividade de espécies: vertebrates
• técnica(s): western blot: suitable
• Isotipo: IgG1κ
• nº de adesão UniProt: P42212
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

28 kDa

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Imunogênio

Epitope: Full Length

Full length GFP protein.

Aplicação

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Ações bioquímicas/fisiológicas

Reacts with most common vertebrate proteins containing the GFP tag.

The antibody reacts with the 28 kDa GFP protein.

forma física

Format: Purified

Protein G Purified

Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Nota de preparo

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Western Blot: GFP recombinant protein

Routinely evaluated by Western Blot on GFP recombinant protein.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable