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Merck

MSIPS4W10

Placa Multiscreen® de 96 poços, membrana hidrofóbica de PVDF

pore size 0.45 μm, sterile

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

Sinônimo(s):

Placa PVDF de 96 poços, Placa hidrofóbica de 96 poços

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Sobre este item

Código UNSPSC:
41104923
eCl@ss:
32039006
NACRES:
NB.22

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Materiais

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Nível de qualidade

descrição

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

esterilidade

sterile

linha de produto

MultiScreen®

Características

hydrophobic
lid

fabricante/nome comercial

MultiScreen®

Parâmetros

50-250 μL sample volume (per well)

técnica(s)

ELISPOT: suitable

área de filtração

0.28 cm2

tamanho da placa

96 wells

volume máximo do poço

300 μL

volume de trabalho

50-250 μL

Matriz

Immobilon®-P

tamanho de poro

0.45 μm pore size

tipo de ligação

high binding surface

Condições de expedição

ambient

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Este Item
MSHVN4BMAIPN4550MSIPN4B10
binding type

high binding surface

binding type

low binding surface

binding type

high binding surface

binding type

high binding surface

material

PVDF membrane (hydrophobic), flat bottom wells, acrylic

material

Barex® , PVDF membrane (hydrophilic), flat bottom wells, titanium dioxide

material

PVDF membrane (hydrophobic), acrylic , flat bottom wells

material

Barex® , PVDF membrane (hydrophobic), flat bottom wells, titanium dioxide

sterility

sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

manufacturer/tradename

MultiScreen®

working volume

50-250 μL

working volume

50-250 μL

working volume

50-250 μL

working volume

50-250 μL

Descrição geral

As placas de membrana de fluoreto de polivinilideno (PVDF) são recomendadas, em vez de um formato de éster de celulose misto, devido à ligação ligeiramente melhorada do anticorpo de captura e ao desempenho superior na detecção de pontos, especialmente para aplicações fluorescentes.

Aplicação

A placa de filtro MultiScreen® HTS IP, 0,45 μm, branca, estéril foi usada no ensaio ELISpot (enzyme-linked immunospot) usando:
  • células plasmáticas produtoras de dsDNA[1]
  • células B de memória e células plasmáticas de vida longa localizadas na medula óssea[2]
  • células de drenagem do linfonodo e do baço

Informações legais

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

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Katrin Klocke et al.
Immunology, 152(1), 125-137 (2017-05-13)
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) -mediated regulation of already tolerized autoreactive T cells is critical for understanding autoimmune responses. Although defects in CTLA-4 contribute to abnormal FOXP3+ regulatory T (Treg) cell function in rheumatoid arthritis, its role in autoreactive T
Mubeen M Mosaheb et al.
Nature communications, 11(1), 524-524 (2020-01-29)
Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of
Leah E Cole et al.
Vaccines, 8(4) (2020-12-06)
While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain
Jing Zhu et al.
Journal of immunology (Baltimore, Md. : 1950), 204(5), 1091-1100 (2020-01-29)
Almost a decade has passed since the approval of belimumab, an mAb directed against B lymphocyte stimulation and the first targeted therapy approved for systemic lupus erythematous (SLE) in over 50 y. Although well tolerated, the efficacy of belimumab remains

Artigos

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocolos

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Conteúdo relacionado

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

MultiScreen® Filter Plates

"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."

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Questions

  1. Do MSIPS4W10 and S5EM077I10 refer to the same plate?

    1 answer
    1. Yes, both MSIPS4W10 and S5EM077I10 correspond to the same plate, identified by the description: MultiScreen HTS IP PVDF 0.45um White Sterile 10pk. MSIPS4W10 is the public catalog number, whereas S5EM077I10 is a custom order request for a specific customer.

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Reviews

Active Filters

  1. Boston, MA
    • Reviews 2
    • Votes 2
    5 out of 5 stars.

    Great plate for ELISPOTS

    These plates give a strong ELISPOT signal with very low background and work well with either a vacuum manifold plate washing apparatus or manual washing.

    Helpful?

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