Merck
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61135

Sigma-Aldrich

Crystal Violet

for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Número do índice de cores:
42555
Beilstein:
3580948
Número MDL:
ID de substância PubChem:
NACRES:
MA.02

grau

for microscopy (Bact., Bot., Hist., Vit.)
indicator (pH 0.1-2.0)

Nível de qualidade

200

forma

powder

visual transition interval

0.1-2.0, yellow-green to blue-violet

pf

205 °C (dec.) (lit.)

densidade

1.190 g/cm3

ε (extinction coefficient)

≥1750 at 585-595  nm in water

adequação

suitable for microscopy (Bact., Bot., Hist., Vit.)

espectro de atividade do antibiótico

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Modo de ação

cell membrane | interferes
enzyme | inhibits

temperatura de armazenamento

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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Descrição geral

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.

Aplicação

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2

Código de classe de armazenamento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificate of Analysis

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Certificate of Origin

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