93362

Trizma^® base

BioUltra, for molecular biology, ≥99.8% (T)

• Sinônimo(s): Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
• Fórmula linear: NH₂C(CH₂OH)₃
• Número CAS: 77-86-1
• Peso molecular: 121.14
• Beilstein: 741883
• Número EC: 201-064-4
• Número MDL: MFCD00004679
• ID de substância PubChem: 57653540
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• grau: for molecular biology
• descrição: aminopeptidase substrate
• linha de produto: BioUltra
• teor: ≥99.8% (T)
• forma: crystalline
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• resíduo de ignição: ≤0.01% (as SO₄)
• perda: ≤0.5% loss on drying, 110 °C
• cor: colorless to white
• pH: 10.5-12.0 (25 °C, 4 M in H₂O)
• useful pH range: 7-9
• pKa (25 °C): 8.1
• pb: 219-220 °C/10 mmHg (lit.)
• pf: 167-172 °C (lit.); 168-172 °C
• solubilidade: H₂O: 4 M at 20 °C, clear, colorless
• traços de ânion: chloride (Cl^-): ≤20 mg/kg; sulfate (SO₄^2-): ≤5 mg/kg
• traços de cátion: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: NC(CO)(CO)CO
• λ: 4 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.10; λ: 280 nm A_max: 0.08
• adequação: in accordance for luminescence
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Descrição

Descrição geral

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.

Aplicação

Trizma has been used:

• In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
• As a component of lysis buffer before immunoprecipitation and immunoblotting
• As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
• As a component of mixed buffer formulations
• As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.

Outras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Easily compare specifications for Trizma products with the Trizma specification table.

Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.

Informações legais

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves