C2910

Complement C3 from human serum

≥3000 C3H50 units/mg (using C3 deficient serum)

• Número CAS: 80295-41-6
• Número MDL: MFCD00130836
• Código UNSPSC: 12352202
• NACRES: NA.61

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• Ensaio: ≥85% (SDS-GE)
• Formulário: solution
• atividade específica: ≥3000 C3H50 units/mg (using C3 deficient serum)
• peso molecular: 185 kDa (disulfide-linked heterodimer)
• técnica(s): activity assay: suitable
• nº de adesão UniProt: P01024
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... C3(718)

Descrição

Aplicação

Complement C3 is important in complement activation through the classical and alternative pathways. Complement C3 has been used in studies of bacterial defense against the complement system. Research on streptococcus infection has shown that Streptococcal pyrogenic exotoxin B (SPE B), a cysteine protease, can prevent phagocytic activity via cleavage of the C3 and impairing the pathways of the compliment system. C3 deficiency can result in recurrent infections and immune complex disorders. Recent research has shown that inherited C3 deficiency can occur either through homozygous mutations or through compound heterozygous mutations,

Ações bioquímicas/fisiológicas

Complement C3 is the third and most abundant component of the complement pathway. It plays a central role in complement activation, being involved in both the classical and alternative pathways. C3 or its proteolytic fragments mediate many biological functions such as opsonization and anaphylatoxin activities. Pro-C3 is synthesized as a large single chain of 185 kDa and is processed to a disulfide-linked heterodimer consisting of an α-chain (115 kDa) and a β-chain (70 kDa) in the plasma. C3 is cleaved by the C3 convertases in either pathway (C4b2a, C3iBb, C3bBb) to C3b (176 kDa) comprised of the large C-terminal portion of the α-chain and the β-chain. C3b interacts with other complement components to initiate the amplification cascade.

Complement C3 is the third and most abundant component of the complement pathway. It plays a central role in complement activation, being involved in both the classical and alternative pathways. C3 or its proteolytic fragments mediate many biological functions such as opsonization and anaphylatoxin activities. Pro-C3 is synthesized as a large single chain of 185 kDa and is processed to a disulfide-linked heterodimer consisting of an α-chain (115 kDa) and a β-chain (70 kDa) in the plasma. C3 is cleaved by the C3 convertases in either pathway (C4b2a, C3iBb, C3bBb) to C3b (176 kDa) comprised of the large C-terminal portion of the α-chain and the β-chain. C3b interacts with other complement components to initiate the amplification cascade. Studies of C3 function have been facilitated by experimentally induced C3 deficiency in animals by injection of cobra venom factor and more recently with gene ablation technology.

forma física

Supplied as a solution in PBS, pH 7.2.

Nota de análise

Functionally pure by a hemolytic assay using deficient sera.

Outras notas

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable