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Merck

D2438

Dimetilsulfóxido

sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Dimethyl sulfoxide

Sinônimo(s):

DMSO

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Sobre este item

Fórmula linear:
(CH3)2SO
Número CAS:
Peso molecular:
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Grade:
BioPerformance Certified
Technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

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Nome do produto

Dimetilsulfóxido, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

SMILES string

CS(C)=O

grade

BioPerformance Certified

agency

USP XXIV
meets EP testing specifications
meets USP testing specifications

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

≤0.1% water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

Quality Level

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Este Item
4164034869472301
Quality Level

400

Quality Level

300

Quality Level

100

Quality Level

200

grade

BioPerformance Certified

grade

ACS reagent, puriss. p.a.

grade

-

grade

ACS reagent

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

-

technique(s)

HPLC: suitable

technique(s)

-

solubility

H2O: miscible (completely)

solubility

-

solubility

-

solubility

-

form

liquid

form

liquid

form

liquid

form

liquid

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

Application

O dimetilsulfóxido foi usado como controle de veículo para cultivar e induzir a ativação de células estreladas hepáticas (HSCs) in vivo.[1][1] Ele também foi usado para preparar uma solução de estoque para estudar a citotoxicidade do ácido ferúlico (FA) nas células do núcleo pulposo (NP).[2]

Disclaimer

Resfria-se intensamente com facilidade e derrete novamente de forma lenta em temperatura ambiente. O produto solidificado pode ser liquefeito novamente aquecendo-o até atingir a temperatura ambiente, sem causar dano ao produto.

Features and Benefits

Produto estéril filtrado pronto para uso embalado convenientemente em frascos âmbar com tampa de rosca e septo. Atende aos requisitos de teste da USP e da EP.

General description

O dimetilsulfóxido (DMSO) é um solvente aprótico. Moléculas terapêuticas e tóxicas que não são hidrossolúveis são frequentemente solúveis em DMSO. Nitrogênio líquido usado juntamente com um agente crioprotetor, como DMSO, é um método amplamente usado para preservar células. O DMSO é usado para preparar solução-estoque de várias moléculas e também é usado como controle para essas moléculas. Ele também foi usado em ensaio de migração celular para dissolver células após a migração. DMSO testado quanto à presença de BPC também atende aos requisitos de aplicações de biotecnologia e engenharia de tecidos.

Classe de armazenamento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies
Phillip McDonagh et al.
Veterinary microbiology, 174(3-4), 438-447 (2014-12-04)
Feline infectious peritonitis (FIP), a feline coronavirus (FCoV) induced disease, is almost invariably fatal with median life expectancy measured in days. Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen
Rory W Blackler et al.
British journal of pharmacology, 172(4), 992-1004 (2014-10-10)
Hydrogen sulphide is an important mediator of gastrointestinal mucosal defence. The use of non-steroidal anti-inflammatory drugs (NSAIDs) is significantly limited by their toxicity in the gastrointestinal tract. Particularly concerning is the lack of effective preventative or curative treatments for NSAID-induced
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many

Artigos

Overview of good cell banking practices for cell line cryopreservation purposes.

Questions

1–10 of 16 Questions  
  1. For D2438-5X10ML, do they come in glass ampoule or bottle with lid?

    1 answer
    1. Yes, both package sizes have a septum cap closure as shown in the image below:

      Helpful?

  2. Hi, what is the difference between DMSO product# D2438 and D2650? What is Hybri-Max vs. BioPerformance certified and meets USP testing specification?

    1 answer
    1. Both D2438 and D2650 are suitable for cell culture. The D2650 is part of the Hybri-Max™ product line. Hybri-Max products have been tested for use in hybridoma creation and culture. The D2438 is part of the BioPerformance Certified group of products. These products are tested and optimized for use in biological applications. For this product and a number of others, this includes testing to meet USP and EP guidelines. Please see the links below to review a sample Certificate of Analysis for each item.
      D2650
      https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation
      D2438
      https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation

      Helpful?

  3. Purity grade of the product ?

    1 answer
    1. The purity of this product is listed on the lot specific Certificate of Analysis. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a Certificate:
      https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation

      Helpful?

  4. El DMSO es sensible a la luz, hay que tomar alguna precaución al respecto?

    1 answer
    1. This product is packaged in amber glass and is protected from light. The 10 mL size is packaged in a sealed ampule. Once opened, any content that is not used should also be stored in amber glass. For long term storage, the material may also be stored under inert gas to preserve the shelf life. Note that this material has a tendency to absorb water. Containers should be tightly sealed.

      Helpful?

  5. what are the storing conditions?

    1 answer
    1. This product is stored at room temperature. Store in a well-ventilated place. Keep cool. Please see the Material Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/d2438.

      Helpful?

  6. What is the difference between products D2438 and D8418?

    1 answer
    1. The D2438 is a BioPerformance Certified grade product suitable for cell culture, hybridoma creation, and cryopreservation. It is intended for research use only but does meet USP and EP testing requirements. The D8418 is a molecular biology-grade product suitable for DNA sequencing, PCR, and transfection. It has been tested for RNAse and DNAse. The products can be selected based on the application.

      Please view the sample Certificate of Analysis for each product to see the testing specifications associated with that item:
      https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF734068.pdf
      https://www.sigmaaldrich.com/coa/SIGMA/D2438/RNBL8837

      Helpful?

  7. What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?

    1 answer
    1. The industry standard for 'endotoxin free' is less than 1 EU/mL.

      Helpful?

  8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

  9. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  10. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

1–10 of 16 Questions  

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