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Merck

E1510

Ethidium bromide solution

BioReagent, Molecular Biology, 10 mg/mL in H2O

Sinônimo(s):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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10 ML

R$ 326,00

R$ 326,00

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Sobre este item

Fórmula empírica (Notação de Hill):
C21H20BrN3
Número CAS:
1239-45-8
Peso molecular:
394.31
Beilstein:
3642536
Número MDL:
MFCD00011724
Código UNSPSC:
41105322
ID de substância PubChem:
24894418
NACRES:
NA.31

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grau

Molecular Biology

linha de produto

BioReagent

concentração

10 mg/mL in H2O

técnica(s)

electrophoresis: suitable

adequação

suitable for gel electrophoresis

cadeia de caracteres SMILES

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

chave InChI

ZMMJGEGLRURXTF-UHFFFAOYSA-N

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Este Item
E1385E763746067
Ethidium bromide solution BioReagent, Molecular Biology, 10 mg/mL in H2O

Sigma-Aldrich

E1510

Ethidium bromide solution

Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

Sigma-Aldrich

E1385

Ethidium bromide solution

Ethidium bromide BioReagent, Molecular Biology, powder

Sigma-Aldrich

E7637

Ethidium bromide

Ethidium bromide solution suitable for fluorescence, ~1% in H2O

Sigma-Aldrich

46067

Ethidium bromide solution

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for fluorescence

concentration

10 mg/mL in H2O

concentration

500 μg/mL in H2O

concentration

-

concentration

~1% in H2O

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

-

Aplicação

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Ethidium bromide solution has been used:
  • as a stain to visualize U937 cells to assess cell viability[1]
  • to detect polymerase chain reaction products[2]
  • in agarose gel electrophoresis based gel retardation assay[3]

Ações bioquímicas/fisiológicas

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Nota de preparo

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Outras notas

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Pictogramas

Skull and crossbonesHealth hazard
GHS06,GHS08

Palavra indicadora

Danger

Frases de perigo

H331,H341

Classificações de perigo

Acute Tox. 3 Inhalation - Muta. 2

Código de classe de armazenamento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
SHBR6796
SHBP1050
SHBM6092
MKCL8014
MKCJ2002

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pH-sensitive oncolytic adenovirus hybrid targeting acidic tumor microenvironment and angiogenesis
Choi JW, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 205, 134-143 (2015)
Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells
Quinones GA, et al.
Blood, 121(7), e25-e33 (2013)
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is
Patrick Seitz et al.
PLoS genetics, 10(1), e1004066-e1004066 (2014-01-07)
The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we
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Conteúdo relacionado

Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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