The solubility of this product in DMSO has not been tested. A 200 mM stock solution can be prepared using ethanol, methanol, and 2-propanol. It is known to have 100 mM solubility in DMSO. The product is insoluble in aqueous solutions. Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C. Storing in a desiccator may be useful.
P7626
Fluoreto de fenilmetanosulfonil
powder, ≥98.5% (GC)
Sinônimo(s):
αFluoreto de α-toluenosulfonil, Fluoreto de benzilsulfonil, Fluoreto de fenilmetilsulfonil, PMSF
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250 MG
R$ 561,00
1 G
R$ 607,00
5 G
R$ 1.042,00
25 G
R$ 3.357,00
100 G
R$ 11.252,00
Sobre este item
Fórmula empírica (Notação de Hill):
C7H7FO2S
Número CAS:
Peso molecular:
174.19
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352202
EC Number:
206-350-2
MDL number:
Beilstein/REAXYS Number:
2088311
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Estamos aqui para ajudarNome do produto
Fluoreto de fenilmetanosulfonil, ≥98.5% (GC)
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
SMILES string
FS(=O)(=O)Cc1ccccc1
biological source
synthetic
assay
≥98.5% (GC)
form
powder
mp
91-94 °C
solubility
dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable
Quality Level
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Este Item | PMSF-RO | 78830 | 52332 |
|---|---|---|---|
| biological source synthetic | biological source - | biological source synthetic | biological source - |
| assay ≥98.5% (GC) | assay 98% (TLC) | assay ≥99.0% (T) | assay ≥99% (GC) |
| Quality Level 300 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form solid | form powder or crystals | form crystalline solid |
| solubility dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable | solubility >10 mg/mL | solubility anhydrous isopropanol: soluble 35 mg/mL, clear to faintly hazy, colorless to faintly yellow (Heating required) | solubility ethanol: soluble, isopropanol: soluble, methanol: soluble |
| mp 91-94 °C | mp 92-94 °C | mp 92-95 °C | mp - |
Application
O PMSF é frequentemente utilizado em tampão de lise para ajudar a preservar as proteínas de interesse durante o isolamento de proteínas e o preparo de amostras por meio da inibição de proteases, que na sua ausência degradariam as proteínas após a lise da célula ou tecido. O PMSF é instável em meios aquosos. Portanto, de modo geral as soluções-estoque de PMSF são preparadas em solventes orgânico anidros (por exemplo, etanol 100% ou isopropanol anidro) antes de serem usadas em meios aquosos.
As características e benefícios do PMSF observadas incluem:
As características e benefícios do PMSF observadas incluem:
- Inibição de proteases serina, como tripsina e quimotripsina
- Também inibe proteases cisteína (reversível por tióis reduzidos) e acetilcolinesterase de mamíferos
- Não é tão efetivo nem tão tóxico como o DFP
- Concentração efetiva 0,1-1 mM
- Meia-vida = 1 h em pH 7,5
O fluoreto de fenilmetanosulfonil foi usado nas seguintes aplicações:
Biochem/physiol Actions
A administração de PMSF produz analgesia não relacionada a seu efeito anticolinesterásico e prolonga o efeito analgésico da β-endorfina administrada centralmente.[5]
General description
O fluoreto de fenilmetanosulfonil (PMSF) é um inibidor amplamente utilizado da protease serina, efetivo contra enzimas como a quimotripsina, trombina e tripsina. O PMSF age como inibidor por meio da sulfonação dos resíduos hidroxila de resíduos serina nos sítios reativos das proteases serina.
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Preços
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Skin Corr. 1B
Classe de armazenamento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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C Pinsky et al.
Life sciences, 31(12-13), 1193-1196 (1982-09-20)
Intraperitoneal (IP) injection of the serine proteinase inhibitor phenylmethylsulfonyl fluoride (PMSF) produced dose-dependent analgesia in Sprague-Dawley rats. AD50 was 2.9 +/- 1.4 (S.E.) mg kg-1, the analgesia was antagonized by naloxone but unaffected by atropine. PMSF significantly enhanced the analgesic
Estrogen receptors activate atrial natriuretic peptide in the rat heart.
Jankowski M, et al.
Proceedings of the National Academy of Sciences of the USA, 98(20), 11765-11770 (2001)
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Finer Y and Santerre JP.
Journal of Dental Research, 83(1), 22-26 (2004)
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The Journal of biological chemistry, 287(36), 30800-30811 (2012-07-19)
Hypoxia-inducible factors (HIFs) are oxygen-sensitive transcription factors. HIF-1α plays a prominent role in hypoxic gene induction. HIF-2α target genes are more restricted but include erythropoietin (Epo), one of the most highly hypoxia-inducible genes in mammals. We previously reported that HIF-2α
Artigos
Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.
Protocolos
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
Conteúdo relacionado
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Can this PMSF powder be dissolved in DMSO, and if yes, then to what concentration where and how to store the reconstituted vial?
1 answer-
Helpful?
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At what temperature should the stock Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 answer-
A 200 mM PMSF solution in dry solvent has been reported to remain active for at least 9 months at
2-8 °C. 100 mM PMSF solutions in isopropanol may be stored at –20 °C for several months. For more information please view the Product Data Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/427/p7626pis-ms.pdfHelpful?
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How should stock solutions of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 answer-
A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.
Helpful?
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What is the effective amount of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), to be used?
1 answer-
The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?
1 answer-
Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.
Helpful?
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What does Product P7626, Phenylmethanesulfonyl fluoride (PMSF), inhibit?
1 answer-
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
Helpful?
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