04-804

Anti-NFAT1 Antibody, clone 8G4.2

clone 8G4.2, Upstate^®, from mouse

• Synonym(e): Nuclear factor of activated T-cells, cytoplasmic 2, NF-ATc2, NFATc2, NFAT pre-existing subunit, NF-Atp, T-cell transcription factor NFAT1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 8G4.2, monoclonal
• Speziesreaktivität: rat, mouse, human
• Hersteller/Markenname: Upstate^®
• Methode(n): immunofluorescence: suitable; western blot: suitable
• Isotyp: IgMκ
• NCBI-Hinterlegungsnummer: NP_001129493.1
• UniProt-Hinterlegungsnummer: Q13469
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NFATC2(4773)

Beschreibung

Allgemeine Beschreibung

120-140 kDa

NFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells.

Immunogen

Epitope: N-term

GST-fusion protein corresponding to amino acids 1-297 of human NFAT1.

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Transkriptionsfaktoren

This Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1.

Biochem./physiol. Wirkung

Detects NFAT1.

Other species have not been tested.

Physikalische Form

Format: Purified

Purified mouse monoclonal in PBS with 0.05% NaN3.

Angaben zur Herstellung

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Hinweis zur Analyse

Routinely evaluated by Western Blot

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable