If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
A4718
Agarose
Molecular Biology
Synonym(e):
3,6-Anhydro-α-L-galacto-β-D-galactan
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Produktname
Agarose, Molecular Biology
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
biological source
algae (marine)
grade
Molecular Biology
form
powder
technique(s)
electrophoresis: suitable
EEO
≤0.05
mp
≤75 °C (3% gel)
transition temp
gel point ≤35 °C (3% gel)
foreign activity
DNase, RNase, NICKase, none detected
storage temp.
room temp
Quality Level
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Verwandte Kategorien
1 of 4
Dieser Artikel | A2576 | A9539 | A6013 |
|---|---|---|---|
| EEO ≤0.05 | EEO ≤0.12 | EEO 0.09-0.13 | EEO 0.09-0.13 |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| grade for molecular biology | grade Molecular Biology | grade for molecular biology | grade - |
| technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable |
| foreign activity DNase, RNase, NICKase, none detected | foreign activity - | foreign activity DNase, RNase, none detected | foreign activity - |
Analysis Note
Sulfatgehalt - dient als Indikator für die Reinheit, da Sulfat die hauptsächlich vorhandene Ionengruppe ist.
Gelstärke - die Kraft, die aufgewendet werden muss, damit ein Gel zerbricht.
Gelierpunkt - die Temperatur, bei der die wässrige Agaroselösung beim Abkühlen geliert. Agaroselösungen zeigen beim Übergang von der Flüssigkeit zum Gel Hysterese - das bedeutet, dass die Temperatur des Gelierpunkts nicht dieselbe ist wie die Schmelztemperatur.
Elektroendosmose (EEO) - Bewegung einer Flüssigkeit durch das Gel. Anionische Gruppen sind in einem Agarosegel an die Matrix gebunden und können sich nicht bewegen, aber dissoziierbare Gegenkationen können in der Matrix in Richtung der Kathode wandern und führen zu EEO. Da die elektrophoretische Bewegung der Biopolymere normalerweise in Richtung der Anode stattfindet, kann EEO die Auftrennung aufgrund von interner Konvektion stören.
Application
Low ethidium bromide and SYBR Green background staining
General description
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is this agarose high-resolution, and suitable for PCR applications?
1 answer-
The High Resolution Agarose, product number A4718, is an intermediate-melting agarose that offers approximately double the resolution capability compared to standard agarose. It is capable of resolving PCR products and DNA fragments ranging from 200-800 bp that differ in size by as little as 2%. This makes it ideal for separating small DNA fragments, such as PCR products, with very small size differences, offering a resolution comparable to that achieved with polyacrylamide gels.
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