14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

• Synonyme(s) : AMPK kinase
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Forme: liquid
• Durée de conservation: 6 mo.
• Poids mol.: M_w 30 kDa (AMPKß1); M_w 38 kDa (AMPKγ1); M_w 64 kDa (AMPKα1)
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable (kinase)
• Solubilité: water: soluble
• Numéro d'accès GenBank: AF100763
• Numéro d'accès NCBI: NM_002733; NM_006253
• Numéro d'accès UniProt: P54619; Q13131; Q9Y478
• Température de stockage: −70°C
• Informations sur le gène: human ... PRKAB1(5564)

Description

Description générale

Research area: Cell Signaling

Product Source: Expressed in E. coli cells and activated in vivo.

Protein Target: AMPK (α1, β1, γ1)

Target Sub-Family: CAMK

Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.[1]

Application

For use in Kinase Assays.

Research Category
Metabolism

Apoptosis & Cancer

Research Sub Category
Diabetes

Obesity

Metabolic Disorders

Actions biochimiques/physiologiques

Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth.[1] Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis.[2] Furthermore, AMPK regulates autophagy and mitophagy.[3] It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.[1]

Conditionnement

Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

Forme physique

Cobalt-agarose

Notes préparatoires

6 months at -70°C

Remarque sur l'analyse

Routinely evaluated by phosphorylation of substrate peptide

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable