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Merck

80457

Acide trifluoroacétique

eluent additive for LC-MS, LiChropur, ≥99.0% (GC)

Synonyme(s) :

TFA

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A propos de cet article

Formule linéaire :
CF3COOH
Numéro CAS:
Poids moléculaire :
114.02
Beilstein:
742035
Numéro CE :
Numéro MDL:
Code UNSPSC :
41116105
Nomenclature NACRES :
NB.21

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Densité de vapeur

3.9 (vs air)

Niveau de qualité

Pression de vapeur

97.5 mmHg ( 20 °C)

Gamme de produits

LiChropur

Essai

≥99.0% (GC)

Forme

liquid

Technique(s)

LC/MS: suitable

Impuretés

≤0.05% water
≤50 mg/kg total sulfur (as SO4)

Indice de réfraction

n20/D 1.3 (lit.)

pb

72.4 °C (lit.)

Pf

−15.4 °C (lit.)

Solubilité

water: soluble 10,000 g/L

Densité

1.489 g/mL at 20 °C (lit.)

Traces d'anions

chloride (Cl-): ≤50 mg/kg
sulfite (SO32-): ≤10 mg/kg

Traces de cations

Ag: ≤0.05 mg/kg
Al: ≤0.1 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.5 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.1 mg/kg
Co: ≤0.05 mg/kg
Cr: ≤0.1 mg/kg
Cu: ≤0.05 mg/kg
Fe: ≤1.0 mg/kg
K: ≤1.0 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤1.0 mg/kg
Mn: ≤0.1 mg/kg
Mo: ≤0.05 mg/kg
NH4+: ≤10 mg/kg
Na: ≤1.0 mg/kg
Ni: ≤0.1 mg/kg
Pb: ≤0.05 mg/kg
Sr: ≤0.05 mg/kg
Tl: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

Absorption UV

λ: 260 nm Amax: ≤0.9
λ: 270 nm Amax: ≤0.1
λ: 280 nm Amax: ≤0.08
λ: 290 nm Amax: ≤0.07
λ: 300 nm Amax: ≤0.06
λ: 320 nm Amax: ≤0.05

Chaîne SMILES 

OC(C(F)(F)F)=O

Adéquation

passes test for LC-MS

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

Clé InChI

DTQVDTLACAAQTR-UHFFFAOYSA-N

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Cet article
T65082995371.08178
grade

eluent additive for LC-MS

grade

-

grade

-

grade

-

assay

≥99.0% (GC)

assay

99%

assay

≥99%

assay

≥99.7% (acidimetric)

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

density

1.489 g/mL at 20 °C (lit.)

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

vapor density

3.9 (vs air)

Description générale

Trifluoroacetic acid is commonly used as a mobile phase additive in liquid chromatography-mass spectrometry (LC-MS).
Features:
  • Extensive QC testing ensures the highest specifications.        
  • Optimized to improve ionization and resolution.        
  • Extremely low levels of inorganic and organic impurities.       
  • Manufactured specifically for accurate and fast LC-MS.

Application

Trifluoroacetic acid may be used as a mobile phase additive for the analysis of basic compounds in biological samples using hydrophilic interaction liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (HILIC–ESI/MS/MS) and reversed-phase liquid chromatography coupled to tandem mass spectrometry (RP-LC-MS/MS).[1]

Conditionnement

Clear borosilicate glass, high hydrolytic resistance (Type I)

Informations légales

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

CorrosionExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

>212.0 °F - Pensky-Martens closed cup

Point d'éclair (°C)

> 100 °C - Pensky-Martens closed cup


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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Simple means to alleviate sensitivity loss by trifluoroacetic acid (TFA) mobile phases in the hydrophilic interaction chromatography?electrospray tandem mass spectrometric (HILIC?ESI/MS/MS) bioanalysis of basic compounds
Shou ZW and Naidong W
Journal of Chromatography. B, Biomedical Sciences and Applications, 825(2), 186-192 (2005)

Articles

Discover the efficient separation six nitrosamine impurities with high reproducibility and precise detection to ensure accurate analysis for regulatory compliance.

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.

Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.

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Protocoles

Aripiprazole, an atypical antipsychotic, treats schizophrenia, bipolar disorder, and major depressive disorder.

Straightforward HPTLC-MS analysis of lactose in dairy products (milk or yoghurt) using only protein crash, centrifugation and dilution as sample preparation.

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

Contenu apparenté

ZipTip® micro-SPE pipette tips are used as a single-step desalting, concentration, and purification tool for complex samples before mass spec analyses.

Questions

1–2 sur 2 questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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