D4522

Deoxyribonucleic acid from calf thymus

Name: Deoxyribonucleic acid from calf thymus
Brand: SIGMA

Type XV, Activated, lyophilized powder

Synonyme(s) :

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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A propos de cet article

Numéro CAS:

91080-16-9

UNSPSC Code:

41106310

eCl@ss:

32160414

EC Number:

293-507-3

NACRES:

NA.52

MDL number:

MFCD00151698

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Nom du produit

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

biological source

bovine pancreas

grade

Molecular Biology

type

Type XV

form

lyophilized powder

solubility

water: slightly soluble

storage temp.

−20°C

Quality Level

200

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Catégories apparentées

Biochemicals

Nucleosides and Nucleotides

Comparer avec des articles similaires

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1 of 4

Cet article	D4764	D8899	D8273
Sigma-Aldrich D4522 Deoxyribonucleic acid from calf thymus	Sigma-Aldrich D4764 Deoxyribonucleic acid from calf thymus	Sigma-Aldrich D8899 Deoxyribonucleic acid, single stranded from calf thymus	Sigma-Aldrich D8273 Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA
grade for molecular biology	grade for molecular biology	grade for molecular biology	grade -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
type Type XV	type -	type -	type -

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

One mg DNA is approx. 20 A₂₆₀ units.

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

0000425610

0000402444

0000402449

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