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Merck

F5803

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Synonyme(s) :

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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A propos de cet article

Formule empirique (notation de Hill) :
C14H16N2O2 · 2HCl
Numéro CAS:
20325-40-0
Poids moléculaire :
317.21
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894894
EC Number:
243-737-5
Beilstein/REAXYS Number:
3917996
MDL number:
MFCD00012488

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Nom du produit

o-Dianisidine dihydrochloride, Suitable for use in glucose determination

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

form

crystalline

pH range

6.95-7.05

mp

268 °C

solubility

water: 2.5 mg/mL, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

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Cet article
D3252D9154D9143
o-Dianisidine dihydrochloride Suitable for use in glucose determination

Sigma-Aldrich

F5803

o-Dianisidine dihydrochloride

o-Dianisidine dihydrochloride ≥95%

Sigma-Aldrich

D3252

o-Dianisidine dihydrochloride

o-Dianisidine dihydrochloride tablet, 10 mg substrate per tablet

Sigma-Aldrich

D9154

o-Dianisidine dihydrochloride

o-Dianisidine peroxidase substrate

Sigma-Aldrich

D9143

o-Dianisidine

solubility

water: 2.5 mg/mL, colorless

solubility

H2O: soluble 25 mg/mL

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

H2O: slightly soluble, aqueous acid: soluble

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

crystalline

form

powder

form

tablet

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

pH range

6.95-7.05

pH range

-

pH range

-

pH range

-

mp

268 °C

mp

268 °C

mp

-

mp

-

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.[1]
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.[2]
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).[3]
  • Myeloperoxidase activity assay.[4]

Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Disclaimer

Probably carcinogenic.

General description

A preweighed vial containing 50 mg o-Dianisidine.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificats d'analyse (COA)

Lot/Batch Number
0000345795
0000345795
SLCM0676
SLCF9565
SLCB2275

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Consulter la Bibliothèque de documents

Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Risheng Ye et al.
The Journal of clinical investigation, 128(3), 1178-1189 (2018-02-20)
The compensatory proliferation of insulin-producing β cells is critical to maintaining glucose homeostasis at the early stage of type 2 diabetes. Failure of β cells to proliferate results in hyperglycemia and insulin dependence in patients. To understand the effect of
G B Penner et al.
Journal of dairy science, 92(6), 2767-2781 (2009-05-19)
The objective of the study was to investigate the fractional rate of volatile fatty acid (VFA) absorption and the expression of genes encoding for transporters and enzymes involved in the absorption and metabolism of VFA in ruminal tissue when cattle
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
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Protocoles

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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