P7119

PGO Enzyme Preparation

Name: PGO Enzyme Preparation
Brand: SIGMA
Price: 198 EUR
Availability: InStock

1 G capsules

Synonyme(s) :

Peroxidase - Glucose oxidase preparation

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10 CAPS

198,00 €

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A propos de cet article

NACRES:

NA.54

UNSPSC Code:

12352204

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Nom du produit

PGO Enzyme Preparation, 1 G capsules

form

powder

solubility

H₂O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

storage temp.

2-8°C

Quality Level

200

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Cet article	345386	P8125	P8375
Sigma-Aldrich P7119 PGO Enzyme Preparation	Sigma-Aldrich 345386 Glucose Oxidase, Aspergillus niger, Recombinant	Sigma-Aldrich P8125 Peroxydase from horseradish	Sigma-Aldrich P8375 Peroxydase from horseradish
form powder	form lyophilized solid	form essentially salt-free, lyophilized powder	form essentially salt-free, lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
solubility H₂O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)	solubility 0.1 M phosphate buffer, pH 7.0: 5 mg/mL	solubility 0.1 M phosphate buffer: soluble (pH 6.0), H₂O: soluble	solubility 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 300

Application

PGO Enzymes preparation has been used:

in diet sampling and chemical analyses[1][2]
to determine plasma and follicular fluid glucose levels[3]
to measure glucose content in fractions of retrograded debranched rice starch[4]

Tested and found suitable for use in the quantitation of glucose.

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Joel I Jokinen et al.

Plants (Basel, Switzerland), 8(6) (2019-06-05)

Infection by holoparasitic plants typically causes decreases in host mass, thought to be primarily as a result of resource abstraction. Inverse relationships have been noted between the number of Orobanche spp. parasites infecting a host and their mass, suggesting that

Evaluation of triticale dried distillers grains with solubles as a substitute for barley grain and barley silage in feedlot finishing diets

K. T. Wierenga, T. A. McAllister, et al.

Journal of Animal Science, 88, 2018-3029 (2010)

A prepartum diet supplemented with oilseeds high in oleic or linoleic acid reduced GnRH-induced LH release in dairy cows during second week postpartum.

Reza Salehi et al.

Reproductive biology and endocrinology : RB&E, 13, 69-69 (2015-07-04)

The objective was to determine the effect of prepartum diets supplemented with rolled canola seed (high in oleic acid) or sunflower seed (high in linoleic acid) on luteinizing hormone (LH) pulsatility and gonadotropin releasing hormone (GnRH)-induced LH release during early

Saturated fat supplementation interacts with dietary forage neutral detergent fiber content during the immediate postpartum period in Holstein cows: Energy balance and metabolism.

P Piantoni et al.

Journal of dairy science, 98(5), 3323-3334 (2015-03-03)

Forty-eight multiparous cows were used in a randomized complete block design experiment with a 2×2 factorial arrangement of treatments to determine the interaction between a highly saturated free FA supplement (SFFA) and dietary forage NDF (fNDF) content on energy balance

Inclusion of wheat and triticale silage in the diet of lactating dairy cows.

M T Harper et al.

Journal of dairy science, 100(8), 6151-6163 (2017-06-12)

The objective of this experiment was to partially replace corn silage with 2 alternative forages, wheat (Triticum aestivum) or triticale (X Triticosecale) silages at 10% of the diet dry matter (DM), and investigate the effects on dairy cow productivity, nutrient

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