R2625
Sinonimo/i:
ATRA, Acido all-trans-retinoico, Acido della vitamina C, Tretinoina
60,70 €
102,00 €
233,00 €
419,00 €
888,00 €
Acido retinoico, ≥98% (HPLC), powder
CC1=C(\C=C\C(C)=C\C=C\C(C)=C\C(O)=O)C(C)(C)CCC1
1S/C20H28O2/c1-15(8-6-9-16(2)14-19(21)22)11-12-18-17(3)10-7-13-20(18,4)5/h6,8-9,11-12,14H,7,10,13H2,1-5H3,(H,21,22)/b9-6+,12-11+,15-8+,16-14+
SHGAZHPCJJPHSC-YCNIQYBTSA-N
synthetic (organic)
≥98% (HPLC)
powder
cell culture | mammalian: suitable
yellow
180-181 °C (lit.)
chloroform: 50 mg/mL
−20°C
human ... RARA(5914), RARB(5915), RARG(5916), RXRA(6256), RXRB(6257), RXRG(6258)
mouse ... Rara(19401), Rarb(218772), Rarg(19411), Rxrb(20182)
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Questo articolo | R4643 | 95144 | 17772 |
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97.5% (HPLC) | assay ≥95.0% (HPLC) |
| form powder | form powder | form (Powder or Powder with Lumps) | form (Powder or Crystal with Lumps) |
| technique(s) cell culture | mammalian: suitable | technique(s) HPLC: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility chloroform: 50 mg/mL | solubility - | solubility - | solubility - |
Danger
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B - Skin Irrit. 2
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK 2
Not applicable
Not applicable
dust mask type N95 (US), Eyeshields, Gloves
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Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
Product Information Sheet
When stored as recommended, the lyophilized retinoic acid is expected maintain stability at least until the recommended retest date printed on the lot specific certificate of analysis:
https://www.sigmaaldrich.com/product/sigma/r2625#product-documentation
Storage of retinoic acid in DMSO at -80c has not evaluated. Protecting the vials from light, and storing the solution under an inert gas will help to extend the stability.
Utile?
Yes, the RA solutions can be filtered with 0.22 uM filters.
The Product Information Sheet also mentions: Perform sterile filtering RA solutions before addition to suspension cells.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/366/983/r2625pis-ms.pdf
Utile?
To assure optimal performance, solutions should be prepared fresh. However, 40 mg/ml in DMSO or 3.0 mg/ml in ethanol (vortexing may be needed) have been stored at -70°C or -20°C for up to two weeks. Solution should be prepared and stored protected from light and under inert gas. Note that aqueous solutions deteriorate quickly.
Utile?
This product is soluble in 95% Ethanol at 2.7mg/mL and in absolute ethanol at 3mg/mL. Please see the link below to review the product datasheet for additional solvents and solution storage information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/231/812/r2625pis.pdf
Utile?
This compound has not been tested for solubility in PEG 1500. Various sources report that retinoic acid is slightly soluble in PEG 400. This information has not been validated. Other solvents include DMSO at 25 mg/mL, ethanol at 10 mg/mL, DMF at 20 mg/mL, chloroform at 40 mg/mL, and acetone at 30 mg/mL. Please see the link below to review the product datasheet which includes additional information on solubility and stability:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/231/812/r2625pis.pdf
Utile?
After dissolving the Retinoic acid in DMSO to create a 20mM stock concentration, I diluted it 1,000 times to achieve a final concentration of 20μM. Crystals have formed in the cell culture dishes where I added this solution but not in the DMSO vehicle dishes. Could there be an issue with this lot, or are there other reasons why crystals are forming?
The product R2625 is soluble at around 40 mg/ml in DMSO, yielding a clear solution. Although the stock seems fine, adding the DMSO solution to the media causes crystals to form. This is common when diluting a high-concentration stock made in organic solvents into aqueous media. To address this, try reducing the stock solution to lower concentrations in DMSO multiple times before adding it to the media to ensure an adequate organic environment around the product. There is nothing wrong with the product itself.
Utile?
The solubility of each Lot of Retinoic Acid is tested in chloroform as shown on the specification sheet.
https://www.sigmaaldrich.com/specification-sheets/150/073/R2625-BULK________SIGMA____.pdf
The Product Information Sheet provides additional solvents, however, water is not recommended:
"RA is more sensitive to light, heat, and air in solution. It is practically insoluble in water; slightly soluble in alcohol and chloroform, sparingly soluble in ether, and soluble in methylene chloride. RA is soluble at about 40 mg/ml in DMSO and at about 2.7 mg/ml in 95% ethanol (vortexing may be needed). "
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/231/812/r2625pis.pdf
Utile?
Stock solutions can be stored at -70 °C for up to two weeks.
Utile?
The 1 G pack size is packaged in a 5 mL glass ampule. This packaging may be opened using ampule breakers. Kindly review the following links: https://www.sigmaaldrich.com/US/en/product/aldrich/z693847 and https://www.sigmaaldrich.com/US/en/product/aldrich/z122890.
Utile?
Yes, this product is soluble in DMSO at 25 mg/mL.
Utile?
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