HPLC Analysis of Mixture of Anisole and Related Compounds on Chromolith^® Performance Diol 100-4.6mm

column

Chromolith^® Performance DIOL, 100 x 4.6 mm (1.53172.0001)

mobile phase

[A] n-Heptane ; [B] 1,4-Dioxane; (95:5, A:B)

flow rate

1.3 mL/min

pressure

203 psi (14 Bar)

column temp.

23 °C

detector

254 nm

injection

5 μL

sample

Anisole 390μg/ml, 3-Nitroanisole 70μg/ml, 4-Nitroanisole 260μg/ml, 2-Nitroanisole 180μg/ml (Diluent: Mobile phase)