CC130

Human Vitronectin

Name: Human Vitronectin
Brand: MM

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

別名:

S-protein, Serum-spreading factor, V75

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この商品について

UNSPSCコード:

12352202

eCl@ss:

32160405

NACRES:

NA.75

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製品名

PluriSTEM-XF^® Recombinant Vitronectin, Human pluripotent stem cell culture.

由来生物

Escherichia coli
human

品質水準

100

アッセイ

≥92% (SDS-PAGE)

フォーム

liquid

メーカー／製品名

PluriSTEM^®

濃度

0.5 mg/mL

テクニック

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

入力

sample type: human embryonic stem cell(s)

UniProtアクセッション番号

P04004

輸送温度

dry ice

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当該品目	V0132	V8379	CC080
Sigma-Aldrich CC130 PluriSTEM-XF^® Recombinant Vitronectin	Sigma-Aldrich V0132 ビトロネクチンラット血漿由来	Sigma-Aldrich V8379 ビトロネクチンヒト血漿由来	Sigma-Aldrich CC080 Vitronectin, Human Purified Protein
biological source Escherichia coli, human	biological source rat plasma	biological source human plasma	biological source human
technique(s) cell attachment: suitable, cell culture \| stem cell: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥92% (SDS-PAGE)	assay -	assay -	assay ≥90% (SDS-PAGE)
concentration 0.5 mg/mL	concentration -	concentration -	concentration 1 mg/mL
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level 100
form liquid	form lyophilized powder	form lyophilized powder	form liquid

詳細

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

アプリケーション

Research Category
幹細胞研究

細胞骨格

ECM＆細胞接着

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

物理的形状

Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.

調製ノート

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.

アナリシスノート

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

法的情報

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

10 - Combustible liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

CC130:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA）を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers.

Mutsumi Kobayashi et al.

Stem cell reports, 17(3), 507-521 (2022-02-13)

In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which

資料

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

HumanKine® Thermostable bFGF

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

プロトコル

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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