Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.
MRCF0R100
Microcon® DNAファストフロー遠心式フィルター
sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)
別名:
Centrifuge concentrator
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この商品について
UNSPSCコード:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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物質
Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)
品質水準
無菌性
non-sterile
特徴
holdup volume> 10 μL
包装
pkg of 100 ea
メーカー／製品名
Microcon®
パラメーター
0.5 mL sample volume
テクニック
DNA purification: suitable
RNA purification: suitable
L
4.50 cm (1.8 in.)
直径
12.3 mm
ろ過面積
0.32 cm2
有効容積
0.5 mL
輸送温度
ambient
関連するカテゴリー
1 of 4
当該品目 | MRCF0R100ET | MRCF0R030 | MRCPRT010 |
|---|---|---|---|
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) |
| feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL | feature holdup volume> 10 μL |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable | technique(s) DNA purification: suitable, RNA purification: suitable, protein extraction: suitable, protein purification: suitable |
詳細
Microcon®遠心式フィルターは、10～500 μLの容量の水性生体サンプルの効率的な濃縮、脱塩、バッファー交換を可能にします。Microcon® DNA FAST FLOWフィルターは、SDSバッファーを用いたゲノムDNAの濃縮や回収に最適化されています。
アプリケーション
特徴および利点
Microcon®遠心式フィルターには、以下のようないくつかの利点があります：
- 逆方向スピンにより、少容量で高回収率を実現
- 低結合率のUltracel®メンブレン
- 迅速な処理
- <20倍の濃縮倍率が得られ、一貫した再現性を確保
その他情報
先発品：42413
法的情報
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Bacillus spp. Bacteriophage and the Dairy Environment
Adamso KL and McKillip JL
Liaoning Chemical Industry, 23(2), 4-4 (2019)
Matteo Berti et al.
Nature communications, 11(1), 3531-3531 (2020-07-17)
Homologous recombination (HR) factors were recently implicated in DNA replication fork remodeling and protection. While maintaining genome stability, HR-mediated fork remodeling promotes cancer chemoresistance, by as-yet elusive mechanisms. Five HR cofactors - the RAD51 paralogs RAD51B, RAD51C, RAD51D, XRCC2 and
関連コンテンツ
Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.
The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.
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Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?
1 回答-
役に立ちましたか？
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The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?
1 回答-
The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:
1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.
2.Sanitization Protocol:
A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.
DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.
役に立ちましたか？
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Has the color of the Microcon DNA Fast Flow become brighter blue?
1 回答-
Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.
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